Fluminense Federal University

About Us The role is that of a Research Assistant in Professor Andrew Tutt’s laboratory within the Breast Cancer Now (BCN) Research Unit, based at the Innovation Hub, Guy’s Cancer Centre, KCL Guy’s Campus. Professor Tutt co-runs an additional laboratory with Professor Chris Lord at the Institute of Cancer Research. As such, the post-holder will be required to have an honorary contract at the ICR and will be required to work flexibly across the two sites; up to 50% of the appointee’s time may be spent at ICR. Professor Tutt’s research group focuses on identifying and characterising novel biomarkers and targets for diagnosis and treatment of breast cancers, respectively. In particular, the group focuses on triple negative breast cancer (TNBC) and breast cancers associated with functional DNA damage repair deficiencies such as BRCA1 and BRCA2 mutations. In recent years, Professor Tutt and Lord’s research group have recently published work in high impact journals including The New England Journal of Medicine, Annals of Oncology, Nature Medicine, Nature Genetics, Cancer Discovery, Nature Communications, Clinical Cancer Research and Oncogene. As a Research Assistant, the post-holder will be required to provide high level technical support to the experimental work being undertaken in multiple projects within Professor Tutt’s laboratories at both KCL and ICR sites. The post holder will be line managed by Professor Tutt, but may at times be supervised in specific areas by other members of Professor Tutt and Lord’s ICR team including Dr Jennifer Trendell (Research Fellow), Dr Valeria Amodeo (Senior Scientific Officer) and Dr Stephen Pettitt (Senior Staff Scientist).The post holder will also work closely with, and in some instances work under the direction of other members of the KCL Breast Cancer Now Unit, including other BCN technical team members. About The Role The post holder’s work will be focused on (but not limited to) working on the following main areas: Processing of patient blood As part of the BTBC study, led by Prof Tutt, we routinely collect blood from breast cancer patients at various timepoints in their treatment pathways. The post holder would be involved in processing blood for use in various projects within the laboratory. From most of these samples, we isolate plasma and immune cells/buffy coat. Plasma is subsequently used for isolation of circulating tumour DNA, which we primarily use for detection of the emergence of BRCA1/2 reversion mutations. From plasma we are also able to isolate extracellular vesicles for projects investigating blood derived biomarkers of breast cancer. Immune cells are used for isolation of germline DNA, co-culture experiments, FACs analysis and in vitro immune cell assays. In addition, whole blood is used for isolation of circulating tumour cells (CTCs) via Parsortix. The post-holder would be involved in processing/extraction of blood samples for all the applications detailed above. Moreover, the post-holder will also be focused on various aspects of the bloods pipeline. This will include maintenance of the Parsortix equipment used for CTC isolation, optimizing the characterization of CTCs isolated from patient blood (this would include immunohistochemical analysis), use of the CellCelector platform, and generation of PDX and PDO models from CTC samples. The postholder will also be involved in projects focused on investigating biomarkers of breast cancer. This may also include isolation of extracellular vesicles (EVs) and characterizing their mRNA and protein cargo via various techniques. Generation of PDO and PDX Models To date, our team have successfully generated a large panel of both PDO and PDX models of breast cancer from patients’ fresh cancer tissues which are subsequently used by the team as representative patient models for translational studies. The main aim of this work is to identify and characterise targets and associated biomarkers for development of novel breast cancer therapies and to investigate response to standard of care therapies with the aim of understanding mechanisms of therapy resistance. The post holder will be involved in establishing and characterizing both PDX and PDO models from patient tumour tissue or needle aspirate samples, patient liquid biopsies (such as pleural effusion and ascites samples) or CTCs isolated from patient bloods. As such the post holder must be prepared to perform both in vitro and invivo studies and will need to hold a Home Office Personal License. PDO Work The post holder will be involved in processing patient samples for PDO model generation, maintenance of 3D cell cultures, genetic perturbation of PDO models via siRNA and CRISPR/Cas9, and analysis of drug response. These experiments will require the post holder to be experienced in the use of echo liquid handler, microplate dispenser, incucyte, CellTiter-Glo and clonogenic survival cytotoxicity assays for performance of dose-response curves and synergy assays. PDX Work The post holder will be involved in processing patient samples for PDX model generation, health monitoring, monitoring of tumour growth and necropsy / tumour dissection. In addition, the post holder may assist with the management of transgenic mouse colonies and drug dosing by multiple routes. As such, the post holder should be proficient in basic in vivo skills The post holder will also be involved in the characterisation of both PDX and PDO models – including DNA, RNA and protein extraction, STR profiling, preparation and submission of samples for sequencing and analysis of samples by qPCR, digital droplet PCR, simple western blot and automated capillary western blot (JESS). This will also entail coordination and interpretation of histology analysis and use of an automated slide scanner. Preparation of patient and PDX/PDO samples for single cell sequencing The post holder will lead on the preparation of samples for single cell sequencing projects. This will involve disaggregation of samples to single cells for storage, managing records of samples stores and coordinating shipment of samples to collaborator sites for single cell sequencing. Providing technical support to various projects within the Tutt/Lord laboratories as required. The post-holder will also provide expert support to Professor Tutt’s laboratory, performing cell culture (2D + 3D), cell function and cytotoxicity assays and generating cell lines/ PDOs with knock down or overexpression of genes of interest (via siRNA or CRISPR/Cas9). The post holder will also be involved in performing protein assays including ELISA, simple western blot and automated capillary based western blot (JESS). For JESS in particular, the post-holder will be responsible for establishing and optimizing protocols for standard use. The post holder will also routinely perform RNA and DNA extraction, qPCR and digital droplet PCR, multi-colour flow cytometry, molecular cloning and microscopy acquisition and analysis. This will include confocal microscopy andmhigh content cell-based imaging (3i Advanced Spinning disk, ImageXpress, Opera Phoenix and tools for imaging quantifications). The successful candidate will also be responsible for the management of the Tutt team’s Incucyte at KCL Guys Campus, providing training and coordinating servicing, maintenance and cost recovery. In addition, the post-holder will be responsible for general laboratory maintenance, including preparation and maintenance of shared stocks and reagents, ordering consumables and reagents, management of equipment, participation in regular laboratory cleaning rota and maintenance of laboratory databases and inventories. They will be required to represent the Tutt KCL BCN Unit Project at ICR laboratory and departmental operational meetings and, where appropriate, should provide training on specialised laboratory skills to junior staff and students. Key relationships The post holder will be required to work in close collaboration with the Unit’s translational research coordinators and clinical fellows, responsible for the acquisition of patient samples. Moreover, they will work closely with members of Professor Tutt’s lab based at both KCL and ICR sites. They will also be expected to establish and maintain collaborative working relationships with members of the KCL based BCN team, led by Dr Sheeba Irshad and the wider Innovation Hub community. In addition, they will also work in close collaboration with ICR based Patient Derived Modelling teams who also focus on generation of PDX and PDO models; the post holder will play a key role in coordinating shipment of patient and PDX/PDO samples to the ICR to facilitate these programmes of work. General Requirements The candidate will be expected to take direct responsibility of specific research projects and will be required to be highly motivated and able to work independently. They will be required to provide original intellectual input and contribute to the design of experiments. They will drive the implementation and optimization of new techniques and be responsible for data analysis, generation of scientific figures and interpretation of results. They will be required to submit regular laboratory reports (currently bi-weekly) summarising their ongoing work, to be reviewed by senior team members. They will also be required to regularly present their work at internal lab meetings and to assist in the preparation of figures, legends and explanatory analysis and methodology text for manuscripts for the experimental work for which they have been involved. The post holder will receive relevant supervision and training in specialised techniques and will meet with senior members of Professor Tutt’s ICR based team on a regular basis to review results and plan experiments. This position is 100% lab-based and requires daily on-site presence (Monday- Friday) during team core-working hours. The candidate will be required to work flexibly across our two laboratory sites: KCL Guys Campus and ICR; up to 50% of the appointee’s time may be spent at ICR. The post holder must be prepared to perform both in vitro and in vivo studies. This is a full time post (35 hours per week), and you will be offered a fixed term contract for two years. Research staff at King’s are entitled to at least 10 days per year (pro-rata) for professional development. This entitlement, from the Concordat to Support the Career Development of Researchers, applies to Postdocs, Research Assistants, Research and Teaching Technicians, Teaching Fellows and AEP equivalent up to and including grade 7. Visit the Centre for Research Staff Development for more information. About You To be successful in this role, we are looking for candidates to have the following skills and experience: Essential criteria BSC (Hons) degree or equivalent in a relevant subject. MSc awarded (or near completion) in a relevant subject is desirable. Extensive experience working in a research role within a target validation laboratory in the cancer field. Proven ability to work independently, to work under pressure to meet deadlines and to manage and prioritise own workload. Experience in the design, conduct, optimisation and analysis of experiments is essential. Able to demonstrate a comprehensive understanding of breast cancer patient subtypes, and the development of targeted therapies in particular those focused on DNA repair deficiencies. Highly experienced and competent at mammalian cell culture. Experience performing cell transfection, drug response experiments, cell-based functional assays and clonogenic survival cytotoxicity assays. Previous experience using liquid handling equipment (i.e. echo liquid handler and microplate dispenser) and use of live cell imaging (i.e. incucyte). Expertise in 3D organoid culture techniques, including experience in performing dose-response curves and synergy assays in organoid setting. Previous experience performing in vivo studies in the cancer setting in rodent models, performing tumour measurements and dissection/necropsy. Previous experience working with patient bloods to isolate plasma and buffy coat/immune cells. Previous experience with germline DNA and circulating tumour DNA isolation from blood. Highly experienced and competent in using protein assays (including ELISA, western blots and automated capillary based western blots). Highly experienced and competent in performing RNA and DNA extractions, PCR, qPCR and digital droplet PCR. Experience of genetic perturbation of cell lines and PDO models, including working with RNAi knockdown systems (including lentiviral work) and CRISPR/Cas9 editing. Desirable criteria Co-authorship of at least 1 peer reviewed publication. Holds a UK Personal Licence for Home Office Modules A, B and C with previous experience working with tumour xenograft and PDX models. Expertise in various dosing techniques for delivery of cancer therapeutics in vivo including intraperitoneal injection (highest priority), oral gavage, IV injection. Experience of cell injections or surgery. Molecular Biology expertise including: DNA manipulation and subcloning Previous experience with extracellular vesicle isolation and circulating tumour cell isolation from bloods. Experience in multi-colour flow cytometry and microscopy acquisition and analysis. Including confocal microscopy and high content cell-based imaging (ie 3i Advanced Spinning disk, ImageXpress, Opera Phoenix and tools for imaging quantifications). Downloading a copy of our Job Description Full details of the role and the skills, knowledge and experience required can be found in the Job Description document, provided at the bottom of the page. This document will provide information of what criteria will be assessed at each stage of the recruitment process. Further Information We pride ourselves on being inclusive and welcoming. We embrace diversity and want everyone to feel that they belong and are connected to others in our community. We are committed to working with our staff and unions on these and other issues, to continue to support our people and to develop a diverse and inclusive culture at King's. As part of this commitment to equality, diversity and inclusion and through this appointment process, it is our aim to develop candidate pools that include applicants from all backgrounds and communities. We ask all candidates to submit a copy of their CV, and a supporting statement, detailing how they meet the essential criteria listed in the person specification section of the job description. If we receive a strong field of candidates, we may use the desirable criteria to choose our final shortlist, so please include your evidence against these where possible. To find out how our managers will review your application, please take a look at our ‘How we Recruit’ pages. Interviews are due to be held at the start of February, exact date TBC. This post is subject to Disclosure and Barring Service and Occupational Health clearances. Grade and Salary: £39,076 – £43,909 per annum, including London Weighting Allowance Job ID: 133965 Close Date: 19-Jan-2026 Contact Person: Jodie Bellamy Contact Details: Jodie.bellamy@kcl.ac.uk

(Fixed term) Liverpool John Moores University (LJMU) is a distinctive, unique institution, rooted in the Liverpool City Region and with a global presence. Our students and staff, past, present, and future, are the beating heart of our city and can be found in every corner of every industry and community. We couldn’t exist anywhere else and have shaped the city in which we belong. Working with the people of Liverpool to improve lives and support communities is at the heart of why we were founded and why we exist today. We are seeking to appoint a Research Fellow to join Prof Mariani’s team (www.mariani-lab.org), based at the LJMU School of Biological & Environmental Sciences, to be a key driver in a new Horizon Europe project: SEA4FUTURE. The research is broadly aimed at improving the sustainability of Mediterranean fisheries and involves 23 institutions in 10 countries. The Liverpool-based team will coordinate work-package 2 – focused on the generation of biodiversity data and complementary interdisciplinary information to enable assessment of fisheries impacts. You will be responsible for generating eDNA metabarcoding data from fisheries across the Mediterranean, entailing the development of an ambitious sampling design, running laboratory experiments using molecular techniques, carrying out bioinformatics and statistical analyses, and leading academic publications. Engagement in outreach initiatives will also be important.  Informal enquires can be made to Professor Stefano Mariani: s.mariani@ljmu.ac.uk Anticipated Interview date (remote): 22nd January 2026  In return, we offer an excellent benefits package including generous annual leave entitlement, pension scheme, induction and development support as well as family-friendly policies. This is an exciting time to join the university as we deliver the LJMU Strategy 2030 and its vision of LJMU as an inclusive civic university transforming lives and futures, by placing students at the heart of everything we do. If you feel that this is the role you have been looking for and your skills and experience can make a real difference at LJMU, we look forward to hearing from you. LJMU is an equal opportunities employer and welcomes applicants from all backgrounds and communities irrespective of age, transgender status, disability, gender, sexual orientation, ethnicity and religion or belief.  All our appointments are made on merit. Please note all of our vacancies will be closed to applications at midnight on the advertised closing date, unless otherwise stated. LJMU are committed to adhering to the Principles set out in the Researcher Development Concordat; in line with this all fixed-term researchers will be supported to complete 10 days professional development activities per year (pro-rata). 

Job Description HONG KONG BAPTIST UNIVERSITY (HKBU) Founded in 1956, HKBU strives to become a world-class research-led University in Asia for the world delivering academic and research excellence in a caring, creative, and global culture.  The University's ambitious Institutional Strategic Plan 2018-2028 sets the research direction to transform HKBU into a reputable international powerhouse for research and teaching excellence.  The University currently has an academic and teaching staff strength of about 1,000, with a total workforce of around 3,200, and a student population of over 13,000.  We offer a broad range of undergraduate and postgraduate programmes through the Faculties/Schools of Arts and Social Sciences, Business, Chinese Medicine, Communication, Creative Arts, Science, and Continuing Education. In Chinese Mainland, the University has successfully pioneered the establishment of a modern liberal arts university, Beijing Normal-Hong Kong Baptist University (BNBU), jointly with Beijing Normal University. To further strengthen research collaborations with universities in Chinese Mainland, the University has set up a number of research centres/institutes such as the Institute of Research and Continuing Education in Shenzhen. LANGUAGE CENTRE The Language Centre is a university teaching unit with the aim to empower HKBU students with competencies in academic, professional, intercultural, and digital communication, preparing them not only for intellectual growth but also for global engagement and career success. The Centre provides diverse learning opportunities in English, Chinese and other languages, including credit-bearing and supplementary courses and activities, and enhancement services. It houses four sections: Chinese, English, Putonghua and Foreign Languages, with a total of about 50 full-time teaching staff and a small team of research/project staff, supported by an administrative team.  Part-time teachers are also engaged. The annual student enrolment in the Centre’s courses and activities is close to 15,000. We are now looking for a visionary, resourceful and dynamic individual who has extensive relevant teaching, research and administrative experience in language education to lead the Language Centre.  Director of Language Centre (at the rank of Principal Lecturer) (25260419) Reporting directly to the Vice-President (Teaching and Learning), the Director will lead the Language Centre to achieve its mission. Responsibilities: Lead and supervise the development and operations of the Language Centre, ensuring alignment with the Centre’s mission and University’s vision and strategic priorities; Oversee the design, development and delivery of the language curriculum and education activities; and collaborate with academic and administrative units in the University, as well as external partners to promote students’ language, communication and intercultural competencies; Drive innovative pedagogic practices in language education, with reference to the latest development in language education research and relevant educational technologies; and collaborate with local, regional and international partners to undertake relevant projects; Monitor students’ language performance and feedback, and use such data to inform the design and delivery of the language curriculum and activities; Supervise all staff in the Centre and oversee its administration; and Engage in university committees. Requirements: A visionary outlook and strong commitment to the holistic development of university students in general; and insights, experience and knowledge in language education in particular; A doctoral degree in a relevant field (e.g., language education and applied linguistics) and at least 15 years of experience including leadership and management roles in language education in the tertiary sector; Excellent leadership, strategic thinking, agility, management and organisational skills, with the ability to work independently and proactively; Excellent interpersonal and communication skills for working with people at different levels, and highly proficient in both written and spoken English, fluency in Chinese (Cantonese and Putonghua) an advantage; and Creativity and openness to integrating innovative pedagogies and technologies into language education initiatives. Shortlisted candidates will be invited to prepare a presentation and a written assessment.  The initial appointment will be offered on a fixed-term contract for three years. Re-appointment thereafter will be subject to mutual agreement and availability of funding.  Salary will be commensurate with qualifications and experience. Application procedures: Applicants are invited to submit their applications at the HKBU e-Recruitment System. Those who are not invited for interview 8 weeks after submission of application may consider their application unsuccessful. Details of the University’s Personal Information Collection Statement can be found at https://hro.hkbu.edu.hk/en/worklife-at-hkbu/employee-favourable-environment.html#privacy-policy. The University reserves the right not to make an appointment for the post advertised, and the appointment will be made according to the terms and conditions applicable at the time of offer. Review of applications is ongoing until the position is filled.