Research Specialist C - Engineering

Pennsylvania (US)
23 Aug 2018
End of advertisement period
23 Oct 2018
Contract Type
Fixed Term
Full Time

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn’s distinctive interdisciplinary approach to scholarship and learning.

Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play.

The University offers a competitive benefits package that includes excellent healthcare and tuition benefits for employees and their families, generous retirement benefits, a wide variety of professional development opportunities, supportive work and family benefits, a wealth of health and wellness programs and resources, and much more.

Penn Engineering’s world-acclaimed faculty, state-of-the-art research laboratories and highly interdisciplinary curricula offer an experience that is unparalleled. Innovation and technology drive every program and transform the fundamentals of what future engineers are learning.

Working at Penn Engineering in the Cremins Lab, the researcher will independently design and conduct moderately complex research in the area of tissue culture experiments on embryonic stem cells, neural progenitor cells, induced pluripotent stem cells and brain organoids. Develop protocols. The researcher will harvest and grow MEF feeder layers. The researcher will perform minipreps and maxipreps to prepare vectors for genome engineering. The researcher will clone sgRNA and CRISPR/Cas9 constructs into standard vector backbones. The researcher will conduct and optimize transfection assays and also prepare and characterize lentiviral particles for genome engineering. The researcher will assist in the long-term culture of whole brain organoids. The researcher will perform clonal selection and screening after transfection. Assist with research supervision and supply ordering.
Position is contingent on continued funding.

A Bachelor’s Degree in molecular and cell biology or related field and 3 years to 5 years of experience or equivalent combination of education and experience is required. Ability to troubleshoot and solve cellular and genome engineering challenges independently and flexibility and independence to take on new/additional technical tasks that may arise during the discovery process will be important.
Position is contingent on continued funding.

Penn adheres to a policy that prohibits discrimination on the basis of race, color, sex, sexual orientation, gender identity, religion, creed, national or ethnic origin, citizenship status, age, disability, veteran status, or any other legally protected class.