The Department of Ophthalmology is seeking a full time Research Technologist. The Research Technologist will work under the general supervision of a faculty investigator, is primarily responsible for managing a Smoking Chamber for experiments looking at the role of cigarette smoking on the eye in a mouse model, and secondarily to help to manage genetically modified mouse colonies. Will perform routine analyses and tests on animal or human fluids and/or tissue or cultured cell lines. Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience. Uses basic understanding of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and trouble-shoot situations needing special attention.
- Work with genetically modified mice that are placed in the smoking chamber and run the smoking chamber
- Maintain and clean the smoking chamber
- Help with breeding, genotyping, and expanding the mice colonies
- Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include: electrophoretic separations, assays, basic molecular biology, DNA and RNA isolation, PCR and RT-qPCR, or other specialized histology and biological science techniques
- Operates basic laboratory equipment such as centrifuge, pH meter, analytical balance, incubator, scintillation counter, spectrophotometer, light microscope, electron microscopy, confocal microscopy, and/or utilizes laminar flow hood
- Operates lab computers for data entry
- Following experimental protocols, monitors animals and maintains health records in accordance with USDA and AALAC guidelines
- Sacrifices and dissects animal remains as required
- Prepares tissue for study utilizing molecular and histochemical techniques
- Bleeds and injects animals according to protocols
- Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports
- Maintains and makes minor adjustments to equipment, e.g. calibrating, alignment
- Complies with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace
- Completes annual university biohazard/universal precaution/radiation safety training, as appropriate
- Uses sterile techniques to avoid contaminating lab experiments
- Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens
- Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Bachelor’s degree, or equivalent, in biochemistry, biology, or related field required. Some related post-undergraduate work experience preferred.
Experience with cell culture strongly preferred. Prefer, but do not require, experience with computer spread sheets (Excel), graphics (Photoshop), word processing (Word). Prefer experience with genome databases and DNA software. Prefer experience in handling radioisotopes. Prefer experience handling laboratory animals (mice and rabbits).
Special knowledge, skills, and abilities:
Must be accurate in work and organized. Must be reliable and willing to learn new skills.
Starting Salary Range: $13.73 - $18.87
Employee Group: Full Time
Schedule: M – F, 40 hours
Employee Subgroup: Non-Exempt
Department Name: Ophthalmology
Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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