UNIVERSITY OF MANCHESTER

Synthetic Biologist/Molecular Biologist/Enzymologist

Location
Manchester, United Kingdom
Posted
28 Jun 2018
End of advertisement period
27 Jul 2018
Ref
S&E-12278
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Fixed Term
Hours
Full Time

We are seeking a talented Synthetic Biologist/Molecular Biologist/Enzymologist to develop new approaches for accelerating the directed evolution of enzymes.

You will work on the design and preparation of synthetic biology-based enzyme mutant libraries. You will ligate and transform these libraries into E. coli cells, and perform FACS analysis, sequencing and enzyme kinetics studies. You will have a relevant PhD in synthetic biology, molecular biology and/or enzymology and experience of mutant library design, ligation, transformation, screening, (next generation) sequencing and enzyme kinetics.

You will work closely with a computational chemist who will be building in silico models of mutant enzymes and integrating them into your predict-design-test-build-learn cycle of directed evolution to allow the most important beneficial mutations to be identified at each iteration. The ability to work both independently and communicate with computational chemists will be essential. In the first instance you will show proof-of-principle on monoamine oxidase, for which we already have extensive experience, and will draw on the excellent infrastructure found within the Manchester Institute of Biotechnology.

The School of Chemistry is strongly committed to promoting equality and diversity, including the Athena SWAN Charter for gender equality in higher education. The School holds a Silver Award which recognises their good practice in relation to gender; including flexible working arrangements, family-friendly policies, and support to allow staff to achieve a good work-life balance. We particularly welcome applications from women for this post. Appointment will always be made on merit. For further information, please visit http://www.chemistry.manchester.ac.uk/about-us/athena-swan/

Please note that we are unable to respond to enquiries, accept CVs or applications from Recruitment Agencies

Enquiries about the vacancy, shortlisting and interviews:

Profs Douglas Kell or Nick Turner

Email: dbk@manchester.ac.uk or nicholas.turner@manchester.ac.uk

General enquiries:

Email: hrservices@manchester.ac.uk
Tel: 0161 275 4499

Technical support:

Email: universityofmanchester@helpmeapply.co.uk
Tel: 0161 850 2004

This vacancy will close for applications at midnight on the closing date