MASARYK UNIVERSITY

Laboratory Technician for the Research Group "RNA-based Regulation of Gene Expression"

Location
Brno (CZ)
Posted
25 May 2018
End of advertisement period
24 Jun 2018
Ref
40469
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Fixed Term
Hours
Full Time

A position for a laboratory technician is available in the research group “RNA-based Regulation of Gene Expression” which is part of
Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating
protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related
biophysical methods.

Research Group Leader: Peter Josef Lukavsky, Ph.D.

Job Description: 

  • Expression and purification of proteins
  • Transcription and purification of RNAs
  • Preparation of various media and buffers
  • PCR and cloning of RNA and protein constructs
  • Preparation of isotope-labelled nucleotides
  • Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis
  • Performance of NMR experiments to study small molecule interactions with proteins and RNA
  • Implementing new techniques and projects
  • Help incoming co-workers into a smooth start in the lab

Requirements: 

  • At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences
  • Good knowledge of English (oral and written)
  • Excellent interpersonal and communication skills
  • High motivation, flexibility and self-driven interest to learn new things
  • Reliability, team player, meticulousness
  • Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.
  • Computer literacy, MS word, Excel.
  • Excellent experience with molecular biology, protein and RNA purifcation, biochemistry and NMR spectroscopy is a big plus

We offer: 

  • Interesting work in a top scientific team
  • Stable base and excellent instrumental equipment of CEITEC MU
  • employment benefits
  • Salary based on qualification and experience; part time appointment possible
     

Brno is an attractive, lively place to live and work with many cultural and recreational amenities.

Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references to Peter Lukavsky (peter.lukavsky@ceitec.muni.cz). Candidates considered for the second round of interview will be informed via email.