MASARYK UNIVERSITY

Postdoc in the field of Call/Structural Biology

Location
Brno (CZ)
Posted
10 Apr 2018
End of advertisement period
30 Apr 2018
Ref
39013
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Permanent
Hours
Full Time

Masaryk University, Brno, Czech Republic invites excellent scientists to apply for a

POSTDOC in the field of CELL/STRUCTURAL BIOLOGY

Joint position Bryja & Damborsky labs

Anticipated start date: The position is available from Aug 2018.

Salary: Unique package with the competitive salary (granted for 48 months)

Future career: Succesful candidate can negotiate junior groupleader position at the Instiute of Experimental Biology, Faculty of Science at Masaryk University

Deadline: The submission deadline is 30th April 2018.

Description:

The project will focus on the biochemical and structural biology analysis of the role of casein kinase 1 delta and/or epsilon (CK1d/e) in the Wnt signaling pathway. CK1 d/e is a crucial but still understudied component of several key developmental signaling pathways - including Wnt/beta-catenin, Wnt/PCP, Hedgehog and Hippo - that are attracting growing attention mainly due to their role in cancer and other human pathologies. Our long term interest in this kinase https://www.ncbi.nlm.nih.gov/pubmed/?term=bryja+v+and+casein+kinase has now resulted in several recent breakthrough discoveries - including validation of CK1 as a therapeutic target in leukemia (Janovska et al., Blood), production of a series of super-selective and subnanomolar dose inhibitors (unpublished) and description of CK1 effects on Dishevelled conformational dynamics uncovered by novel FRET-based system.

The proposed project will build on this work and will aim to combine proteomics, structural and molecular biology to:

  • understand the mechanism of activation of CK1 in the morphogenetic signaling pathways
  • obtain molecular structures of CK1 with the panel of novel inhibitors
  • obtain molecular structures of complexes of full length CK1 with its key target in the Wnt pathway - Dishevelled

The successful candidate should:

The application should include:

A review of applications will commence immediately after the deadline. Short-listed candidates will be invited for interview within one month of the deadline.

Please submit your application (or informal enquiries) by e-mail to bryja@sci.muni.cz.

Background information

Masaryk University (MU) is the second oldest and largest university in the Czech Republic, the most dynamic and research-oriented university. MU has also attained a leading position in competitions for research grants and is making considerable financial investments into its modern university campus to enhance its research capacity. It has effective Technology Transfer Office that supports cooperation between the scientific community and the industry and helps putting research results into practice.

MU offers the opportunity to gain:

Further information about:

Relevant recent literature:

Cervenka I et al.. Dishevelled is a NEK2 substrate controlling dynamics of centrosomal linker proteins. Proc Natl Acad Sci USA. 2016;113(33):9304-9.

Janovska P, Poppova L, et al. Autocrine signaling by Wnt-5a deregulates chemotaxis of leukemic cells and predicts clinical outcome in chronic lymphocytic leukemia. Clinical Cancer Research 2016 22(2):459-69.

Janovska P, et al. Casein Kinase 1 is a Therapeutic Target in Chronic Lymphocytic Leukemia. Blood 2018; 131(11):1206-1218

Vanacek, P. et al., 2018: Exploration of Enzyme Diversity by Integrating Bioinformatics with Expression Analysis and Biochemical Characterization. ACS Catalysis 8: 2402–2412.

Marques, S. M., Daniel, L., Buryska, T., Prokop, Z., Brezovsky, J., Damborsky, J., 2017: Enzyme Tunnels and Gates as Relevant Targets in Drug Design. Medicinal Research Reviews 37: 1095-1139.