PhD Scholarship in Biopharmaceutical Discovery and Advanced Manufacturing

Location
Brisbane, Australia
Posted
11 Sep 2017
End of advertisement period
31 Oct 2017
Ref
501158
Contract Type
Fixed Term
Hours
Full Time

The Australian Institute for Bioengineering and Nanotechnology

Isolation and characterisation of novel antibodies against intra-cellular target

The University of Queensland's Australian Institute for Bioengineering and Nanotechnology (AIBN) is a dynamic multi-disciplinary research institute dedicated to developing technology to alleviate societal problems in the areas of health, energy, manufacturing and environmental sustainability. AIBN brings together the skills of more than 250 world-class researchers complimented by an extensive suite of integrated facilities, working at the intersection of biology, chemistry, engineering and computer modelling. With a reputation for delivering translational science, AIBN conducts research at the forefront of emerging technologies, and has developed strong collaborations with leading members of industry, academia and government. AIBN goes beyond basic research to develop the growth of innovative industries for the benefit of the Queensland and Australian economies.

Information about the Institute can be accessed on the Institute’s web site at http://www.aibn.uq.edu.au/.

The ARC Training Centre for Biopharmaceutical Innovation (CBI), located within the AIBN is enhancing Australia’s biopharmaceutical capabilities through dedicated research and development programs with leading industrial partners. The Centre conducts research in three thematic areas:  new biopharmaceutical and diagnostic agent discovery; development of mammalian cells as factories for recombinant protein production; and advanced manufacturing of biopharmaceuticals. The CBI is supported by industry partners CSL Limited, GE Healthcare, the Australian Red Cross Blood Service (ARCBS) and Patheon Biologics Australia Pty Ltd.

As a condition of the ARC Industrial Transformation Training Centre funding scheme, students will spend periods of time at the CSL Research Laboratories at Parkville, Melbourne, that total at least 12 months over a four year period. 

The project

Project that will offered in collaboration with CBI partner CSL:

Isolation and characterisation of novel antibodies against intra-cellular target

The overarching goal of the work is to establish the proof of principle that innovative delivery methods enable the targeting of protein activity even inside cell nuclei. The candidate will be housed in an excellent research environment with the latest laboratory facilities for antibody discovery and recombinant protein expression in mammalian cells.

The project will be articulated in 2 parts:

  1. To develop methodologies for delivery of monoclonal antibodies of therapeutic significance that target transcription factor activity. The successful candidate will receive systematic training in molecular biology, cell biology and more specifically antibody engineering. The candidate will also receive training in methods for antibody characterisation including plasmon surface resonance, fluorescence activated cell sorting and various in vitro and in vivo bioassays and advanced microscopy. 
  2. To validate transcription factor inhibition in vitro and in vivo: the aim is to identify and characterise the inhibition of a transcription factor activity via scfv fragment delivered to cells using novel cutting-edge method. The successful candidates will receive systematic training in molecular and developmental biology as well as genomics, and apply these techniques to validating the inhibition of transcription factor activity.  The candidate will also receive training in techniques for culturing cells at small scale, and in the analysis and characterisation of zebrafish vascular development using established transgenic lines. 

The candidate will be housed in an excellent research environment with the latest laboratory facilities for recombinant protein expression in mammalian cells and protein characterisation.

The persons

A PhD is about learning new skills, how to do research, and becoming an independent researcher. We recognise that students will come to us from different backgrounds in order to learn these new skills and we welcome interest from domestic (i.e. Australian and New Zealand) students and international students.

Applicants will meet the requirements of admission to a Research Higher Degree at UQ, as described at https://graduate-school.uq.edu.au/uq-research-degrees. This includes evidence of one of the following with a background in molecular biology, protein chemistry or chemical engineering:

  • A bachelor's degree with honours class IIA or better from approved universities, which should include a relevant research component.
  • A research master's degree.
  • A coursework master's degree and an overall GPA (grade point average) equivalent to 5.65 on the 7-point UQ scale, which should include a relevant research component.
  • A postgraduate degree of at least one year full-time equivalent with an overall GPA (grade point average) equivalent to 5.0 on the 7-point UQ scale, together with demonstrated research experience equivalent to honours IIA will be considered for PhD entry on a case by case basis.
  • A bachelor's degree plus at least two years of documented relevant research experience, including research publications.

English language proficiency requirements apply.

Mandatory requirements for international applicants:

  1. At least 2 high quality research publications;
  2. Excellent academic performance evidenced by a high Grade Point Average (GPA); and
  3. International applicants must meet the University of Queensland's English Language Proficiency (ELP) requirements, detailed at http://www.uq.edu.au/grad-school/english-language-proficiency-requirements

Remuneration

The base stipend will be at the rate of AUD $26,682 per annum tax-free for three years with two possible six month extensions in approved circumstances.

Enquiries

To discuss this role please contact Associate Professor Mat Francois via email m.francois@imb.uq.edu.au.  

To apply for this role please use the Apply button below and quote the Project Title above in your Cover Letter.

Application closing date

31/10/17