Postdoctoral Position - Protein Engineer

Bordeaux (FR)
22 Feb 2017
End of advertisement period
15 Apr 2017
Contract Type
Fixed Term
Full Time

Postdoctoral Position - Protein engineer
European Institute of Chemistry and Biology
INSERM U1212, CNRS UMR 5320, Bordeaux, France

A postdoctoral position for a protein engineer is available in the lab of Dr. Axel Innis (www.innislab.org) at the European Institute for Chemistry and Biology (IECB) in Bordeaux (http://www.iecb.u-bordeaux.fr). We are seeking a highly motivated candidate with a PhD in Protein Engineering or in a related field, an excellent track record and fewer than 2 years postdoctoral experience. Proficiency in molecular biology techniques for nucleic acids
(DNA/RNA) is a must. Moreover, we are particularly interested in candidates with strong expertise in directed evolution coupled to in vitro compartmentalization and/or using deep sequencing as readout. Candidates should speak English fluently.

The project will make use of the bacterial ribosome as a high-throughput selection platform for the directed evolution of novel antimicrobial peptides (Seefeldt et al. (2015) Nat Struct Mol Biol 22, 470-475). The successful applicant will adapt our recently developed inverse toeprinting selection strategy to the screening of peptide sequences that block the bacterial ribosome in trans, in collaboration with the droplet-based microfluidics group of Prof. Jean-Christophe Baret. The peptide inhibitors of translation identified by this method will be further characterized through biochemical and structural means, and results from the project will directly contribute to developing antimicrobials that target multi-drug resistant pathogens.
The IECB is a thriving interdisciplinary research institute located on the campus of the University of Bordeaux, in the southwest of France. It is equipped with state of the art facilities for molecular biology, biochemistry, structural biology (cryo-EM, X-ray, NMR and molecular modeling), mass spectrometry, imaging and other biophysical techniques. The Innis lab
provides a highly dynamic international environment, where cutting edge research on bacterial  translation and its regulation by peptides and antibiotics is carried out using structural, molecular and computational biology approaches, including directed evolution techniques.

The start date is flexible, but no earlier than June 1st, 2017. This position is full time, 38.5 hours per week, with an initial fixed term of 1 year that can be further extended. The initial gross salary will begin at 30,352 euros per year and will be dependent on the skills and experience of the successful applicant. Funding for this position will be provided through an ERC
Consolidator grant (Project acronym: NascenTomiX).

Candidates interested in applying for this position should send a cover letter
and a CV describing their research experience and publication list to Dr.
Axel Innis (axel.innis@inserm.fr) by April 15, 2017. They should also provide
the names and contact details for at least two referees.