Research Technician

California University of Technology
Pasadena, California, United States
28 Sep 2016
End of advertisement period
26 Oct 2016
Contract Type
Full Time

Job Summary

We have secured funding for a 2-year project investigating the use of a novel immunotherapy approach to treat HIV infection. This approach relies on modifying hematopoietic stem cells and testing them in humanized mouse models.

This project involves engineering human T cells and Hematopoietic stem cells to develop an immunotherapy for HIV infection. The engineered T cells and HSCs will be tested in vitro and in humanized mouse models for their efficacy in controlling HIV infection.

The primary purpose of this position is to perform research for this project under the supervision of Dr. David Baltimore and Dr. Alok Joglekar. The primary responsibility is to design and conduct in vitro and in vivo experiments, to assist in experiments, and to keep detailed records of research. The research involved working with viral vectors, human primary cells, HIV, and mouse models.

Job Duties

  • Research involves making and testing lentiviral, retroviral and Adeno-associated virus vectors in human T cells and HSCs. The technician will perform the required cell culture, vector production and testing, titering by qPCR or flow cytometry.
  • Research also involves performing in vitro assays using T cells, human cell lines, and HIV-infected cells. The technician will perform the required cell culture, in vitro viral infections, and flow cytometry.
  • Research also involves performing experiments with humanized mice reconstituted with human cells. The technician will perform the required cell processing to establish humanized mouse models, harvest samples from these mice, and perform flow cytometry and other in vitro assays.
  • Research involves performing high-throughput sequencing on various samples. The technician will construct libraries for sequencing, analyze sequencing data, and perform the required molecular biology techniques
  • Other duties as assigned

Basic Qualifications

  • Bachelor’s degree or equivalent in biology, biochemistry, or related field.
  • At least 3 years of full time experience in biological research.
  • Must have prior experience in: culture of human cells, transfections, flow cytometry
  • Must have experience in basic molecular biology techniques such as PCR, plasmid isolation
  • Must be highly motivated, productive, and organized.
  • Must have excellent interpersonal skills – ability to interact well with other lab members.

Preferred Qualifications

  • Doctoral degree or equivalent in biology, or equivalent
  • Experience on working with viruses or viral vectors under BSL2/2+ conditions
  • Experience in high throughput sequencing such as Illumina
  • Experience in bioinformatics techniques
  • At least 2 years of relevant work experience preferred.

Supervisory position? No

Posting Detail Information

Posting Open Date 08/10/2016
Posting Close Date
Open Until Filled Yes

EEO Statement

We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, or national origin, disability status, protected veteran status, or any other characteristic protected by law.