Job Description

A research assistant position is available immediately in Dr Anand Jeyasekharan’s lab at the Cancer Science Institute of Singapore, for a motivated and experienced scientist to drive a project investigating the determinants of immune activation after DNA damage in cancer.

Key Responsibilities

• Perform wet lab-based experiments pertaining to the project, being responsible for project progression.
• Compile, interpret and present generated data at lab meetings and conferences.
• Manage collaborations with academic and industry partners where necessary.
• Assist with management of lab including but not limited to procurement and safety matters.

Qualifications

Essential requirements

• A degree in Biology (Cellular/Molecular biology/ Genetics/ Biochemistry), minimally a bachelor’s with honours.
• Expertise in the following molecular biology techniques:

Mouse handing experience including:

•  Genotyping (mouse management and breeding).
•  Organ harvesting and digestion for downstream analysis.
• Tumour inoculation by subcutaneous and intravenous injection.
• Blood collectionn

Mammalian cell culture

• Experience with both adherent and suspension lines.

Flow cytometry:

• Experience with multi-colour flow cytometry and high-dimensional analysis ( e.g UMAP and tSNE).
• Experience with immunophenotyping of mouse tissue.
• Ability to communicate fluently in written and spoken English
• To be a team player with the ability to work collaboratively with other members of the lab.
• At least 3 years’ experience

Preferential requirements

• Expertise in the following molecular biology techniques:
• Routine molecular techniques including but not limited to sub-cellular, fractionation, Western blotting, immunoprecipitation, qPCR, cell viability assays, cell stimulation, drug treatment and sphere forming assays.
• Experience with cell manipulation such as gene knockdown or overexpression.
• Experience with primer design, cloning and viral transduction would be an added advantage.
• Immunofluorescence staining and confocal microscopy experience.
• Proficiency in basic laboratory statistical software (PRISM, SPSS or similar).
• Experience in laboratory management.

Interested applicants, please email your CV to Dr Anand Jeyasekharan at csiadj@nus.edu.sg . In your CV, please include contact information and at least two referees.

Further information on the laboratory and institute can be found at:https://www.csi.nus.edu.sg/web/anand-jeyasekharan/

Covid-19 Message

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Taking into consideration the health and well-being of our staff and students and to better protect everyone in the campus, applicants are strongly encouraged to have themselves fully COVID-19 vaccinated to secure successful employment with NUS.