Research Assistant/Research Associate

Location
London (Greater)
Salary
Research Assistant £34,502-£39,333 / Research Associate £40,386-£47,414 per annum, including London
Posted
30 Sep 2022
End of advertisement period
16 Oct 2022
Ref
055568
Academic Discipline
Clinical, Pre-clinical & Health
Job Type
Research Related
Contract Type
Fixed Term
Hours
Full Time

Job description

The post holder will be responsible for producing genomic DNA/RNA from patient/control tissue samples and performing necessary quality controls/molecular measurements therein. Organization of samples and performing further processing steps as required. Performing specified genomic tests, including next generation sequencing-based gene panel screening and single cell sequencing to map patient specific mutation profiles, and TCR sequencing to investigate T cell receptor clonotype behaviour. Undertaking advanced molecular biology techniques as required to substantiate and follow-up the initial findings, such as molecular barcode-based PCR and ddPCR to verify panel and single-cell findings. Ensuring that required materials for the laboratory procedures are ordered and ready for use when they are needed. Manipulation of primary human cells using aseptic technique and downstream sorting of the cells via MACS or flow cytometry. Bioinformatic analysis of these data sets, using R, bash, and Python, presentation of data in group and departmental meetings and preparing draft manuscripts based on the results obtained.  

 

This post will be offered on a fixed-term contract until 30th November 2023 

This is a full-time post – 100% full time equivalent

 

Key responsibilities

  • Completing molecular biology protocols in the laboratory including NGS library preparation, and both flow and column sorting of cells  
  • Analyse results with appropriate bioinformatics tools 
  • Maintain organization of laboratory work area and order reagents in as required 
  • Communication of results to supervisor and other research groups in the department 
  • Management of own research, planning, developing and executing experiments effectively to deadlines 
  • Undertaking other duties that are commensurate with the grade and responsibilities of the post, including contributing to activities related to the organization and day to day activities of the lab. 

The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.  

Skills, knowledge, and experience 

Research Assistant (Grade 5)  

Essential criteria

1.      Hold a relevant undergraduate or master’s degree or be close to completion 

2.      Experience in basic molecular biology techniques including NGS library preparation and sequencing and PCR 

3.      Evidence of significant academic attainment and interest in the area 

4.      Ability to manage own academic research and associated activities 

5.      Experience of working in a research team 

6.      Excellent communication skills, and ability to describe clearly scientific data 

 

Desirable criteria

1.      Experience in single cell as well as TCR-Seq NGS library preparation. 

2.      Experience in multi-colour flow cytometry 

3.      Experience in magnetic column cell separation 

4.      Experience in working with clinical trial samples/GCP 

 
 

Research Associate (Grade 6)

Essential criteria

1.   PhD in genomic or immunology related discipline  

2.   Independency to troubleshoot, evaluate and design protocols  

3.   Critical thinking   

 

Desirable criteria

1.    Experience in single cell as well as TCR-Seq NGS library preparation. 

2.    Experience in multi-colour flow cytometry 

3.    Experience in magnetic column cell separation 

4.    Experience in working with clinical trial samples/GCP 

 

Please note that a research associate is a PhD level role but candidates who have submitted their thesis and are awaiting award of their PhDs will be considered. In these circumstances the appointment will be made at Grade 5, spine point 30 with the title of Research Assistant. Upon confirmation of the award of the PhD, the job title will become Research Associate and the salary will increase to Grade 6. 

 

Further information

Professor Mufti’s group is part of a research programme at the Comprehensive Cancer Centre dedicated to investigating bone marrow failure syndromes, include myelodysplastic syndrome and aplastic anaemia. The other groups lead focused on blood cancers include: BMS Genetics Group Lead (Dr Lynn Quek), BMS Immunology Lead (Dr Giorgio Napolitani), Dr Mohammad Karimi, BMS Bioformatics Lead, Leukaemia Stem Cell Biology (Professor Eric So), Gene & Cell based Therapies (Professor Farzin Farzaneh), CAR-T Cell Programmes (Dr Reuben Benjamin), post-transplant immunotherapies (Professors Francesco Dazzi, Dr Pramila Krishnamurthy and Dr. Victoria Potter) and King’s College Hospital Haemato-oncology Molecular Diagnostics Centre (Dr Deborah Yallop, Dr Shireen Kassam and Dr Guy Hannah). These research programmes benefit from an extensive Biobank of blood cancers, and a recent investment in infrastructure and new equipment. Research groups at Denmark Hill will have on-site access to a BD Aria Fusion Flow Sorter, BD Lyric Flow Analyzers, a Helios CyTOF, an Illumina NextSeq Sequencing System, 10x Genomics Chromium platform and automated sample and nucleic acid handling systems. These research programmes are closely linked to clinical services, where research activities are translated into novel therapies including CAR-T cells/immunotherapies (with specific emphasis on allogenic CART-T clinical trials in Lymphomas/ALL & AML).  

 

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