NATIONAL UNIVERSITY OF SINGAPORE

Research Assistant, COE Project

6 days left

Location
Singapore
Posted
12 Sep 2022
End of advertisement period
12 Oct 2022
Ref
10099844
Contract Type
Permanent
Hours
Full Time

Job Description

Fluorescent labels are essential tools in many branches of life science research. They provide high sensitivity for detection and are non-destructive to the target samples. Traditionally, fluorescent labeling is achieved by adding a chromophore to biomolecules such as proteins or nucleic acids. Conjugated oligoelectrolytes (COEs) are emerging as a new class of fluorescent molecules used to label cells and other specimens for non-invasive fluorescence imaging directly

COEs are defined by a linear hydrophobic conjugated backbone with polar ionic pendants at both terminals. They were firstly synthesized in Prof. Bazan’s group as two-photo absorption chromophores and later found to have biological applications. COEs are membrane intercalating molecules that span the lipid bilayer. The spontaneous intercalation into membranes from aqueous media results in significant increases in fluorescence emission. By tuning the molecular framework in the conjugated core of COE, COEs characterized by different emission wavelengths can be achieved, thereby gaining access to a range of different colors for fluorescent labeling.

This research aims to determine the labeling efficiency of various COE constructs across different biological samples, including but not limited to mammalian cells, bacteria of different membrane structures, and extracellular vesicles. The characterization and applications for these COE-labelled samples will also be investigated via different fluorescence-based techniques such as confocal microscopy, flow cytometry, and in vivo fluorescent imaging.

Qualifications

  • The candidate must have a Bachelor Degree with Honours or its equivalent 
  • Independent and motivated individual with good problem-solving skills 
  • With practical experience and/or knowledge of:
    • Biological chemistry, cell biology, or biochemistry.
    • Characterization techniques including absorbance/fluorescence measurements, flow cytometry or fluorescent microscopy
    • Microsoft Office

Interested applicants may email your resume to Prof. Bazan (chmbgc@nus.edu.sg) and Sarah (chmsjc@nus.edu.sg)

Covid-19 Message

At NUS, the health and safety of our staff and students are one of our utmost priorities, and COVID-vaccination supports our commitment to ensure the safety of our community and to make NUS as safe and welcoming as possible. Many of our roles require a significant amount of physical interactions with students/staff/public members. Even for job roles that may be performed remotely, there will be instances where on-campus presence is required.

Taking into consideration the health and well-being of our staff and students and to better protect everyone in the campus, applicants are strongly encouraged to have themselves fully COVID-19 vaccinated to secure successful employment with NUS.

More Information

Location: Kent Ridge Campus
Organization: Science
Department :Chemistry
Employee Referral Eligible: No
Job requisition ID : 16842

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