This is an exciting and unique opportunity for a research assistant to help set up the newly established Nolan Hearing Lab, funded by the Wellcome Trust. The overarching objective of the research programme is to understand the molecular mechanisms that underlie the long-known sex-differences in the maintenance of hearing over the life course and determine if sex-specific molecular pathways can be identified that are suitable as potential drug targets in age-related / adult-onset hearing loss (ARHL).
The Nolan Hearing Lab is based within the Wolfson Centre for Age-Related Diseases (Wolfson CARD), which forms part of the world-renowned Institute of Psychiatry, Psychology & Neuroscience at King’s College London. Research at the Wolfson CARD spans a wide remit across three main Research Themes: Chronic Pain and Migraine; Brain and Spinal Cord Repair; Hearing Loss and Sensory Systems.
The research assistant will work in the lab of Dr Lisa Nolan and will be responsible for maintaining colonies of genetically modified mice, including reporter lines and mice carrying targeted deletions in the inner ear. The post-holder will also be responsible for routine housekeeping tasks in the lab (stock checks, ordering, maintenance of equipment) and provide support for the molecular and histology work undertaken. Responsibilities will also include assisting with specialist auditory brainstem response recordings in genetically modified mice, immunofluorescence and confocal microscopy imaging. Experience in mouse work and an active Home Office Personal Licence is desirable.
The position will be offered fixed-term for 1 year initially, with potential for extension beyond this.
Informal enquires about the post are most welcomed - please contact the PI directly. Interviews are expected by mid-October. The start date is as soon as possible, but this is negotiable.
This post will be offered on an a fixed-term contract for 1 year initially.
This is a full-time post - 100% full time equivalent
- Management of mouse colonies - breeding genetically modified mice to generate required genotypes, ear notching litters and genotyping.
- Dissection, preparation and cryosectioning of mouse cochlear tissue.
- Dissection and preparation of mouse cochlear tissue for whole mount and electron microscopy as required.
- Immunofluorescence and confocal microscopy imaging.
- To assist with auditory brainstem response recordings in genetically modifiedmice.
- Maintain up-to-date, accurate and comprehensive laboratory notes.
- To analyse and process data to produce figures suitable for publication.
- Active participation in and presenting data at regular lab meetings.
- Organization and day-to-day management of housekeeping laboratory duties.
- Assist, supervise and train other members of the team, including students and other research staff on related projects.
The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.
Skills, knowledge, and experience
BSc in Molecular Biology/Genetics, Neuroscience or a related subject area.
Basic understanding of genetics.
Experience in laboratory research
Previous molecular biology or histology experience (eg: PCR, Western blot /cryosectioning)
Excellent and demonstrated ability to work in a team.
Willingness to work with mouse mutants.
Record keeping and experimental planning - excellent organizational skills and meticulous attention to detail.
Ability to multitask, plan and prioritise workload to meet deadlines.
Ability to work independently and troubleshoot technical problems.
Excellent written and verbal communication skills.
1. MSc / MRes in Molecular Biology/Genetics, Neuroscience or a related subject area.
2. Home office personal licence
3. Experience working with small rodents
4. Experience performing auditory brainstem response recordings.
5. Experience of communicating with people who have different types of hearing loss.
Informal enquires about the post are most welcomed - please contact the PI directly and quote the job reference number. Dr Lisa Nolan: firstname.lastname@example.org
Interviews are expected by mid-October. The start date is as soon as possible, but this is negotiable.