Research Assistant for The APIPPRA Biomarker Project (TAB-P)
Job purpose: We seek to recruit an enthusiastic research assistant who, on a day-to-day basis will coordinate the APIPPRA Biomarker project. This project involves analysis of a large archive of biological material acquired from study participants recruited to the APIPPRA study, a prevention clinical trial targeting individuals at high risk of developing rheumatoid arthritis (RA). Samples include genomic DNA, RNA derived from whole blood and PBMC, PBMC, serum and urine.
Responsibilities: The successful candidate will be responsible for managing the archive of APIPPRA study samples, and for coordinating shipment of samples from the UK Biocentre in Milton Keynes to collaborators and external vendors. In addition, the research assistant will work closely with postdoctoral research associates in the laboratory developing and rolling out assays designed to evaluate immune signatures of subjects at risk of RA. This will include immune phenotyping by multi-dimensional flow, spectral and mass cytometry, as well as high throughput of immune assays designed to detect peptide reactive T and B cells. Experience in the analysis of biological material at the mRNA level would be an advantage.
Training opportunities: The successful candidate will join a team of experienced cellular and molecular immunologists who have expertise in the immunogenetics and immunobiology of rheumatoid arthritis. Working in a large state-of-the-art laboratory based on Guy’s campus, training will focus on aspects of immune phenotyping and the use of novel analytical tools for the detection of immune signatures of the “at risk” phenotype.
This post will be offered on a fixed-term contract for 3 years
This is a full-time post - 100% full time equivalent
• To oversee of a large archive of biological material, underpinned by HTA guidelines and procedures for biobanking
• To coordinate shipment of all samples to collaborators and vendors, accompanied by detailed sample manifests
• Performing and optimising specific cellular and molecular techniques relevant for the project including flow cytometry, cell-based immunology assays and gene expression techniques
• Performing routine housekeeping tasks including lab stock checking, ordering lab materials, preparing buffers, aliquoting reagents, waste management, looking after communal equipment and facilities
• To support other members of the team and when needed provide supervision of BSc/MSc students
• Performance of any other reasonable duties including administrative support where required (e.g. participation in internal HTA and equipment committee meetings)
The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.
Skills, knowledge, and experience
• A flexible team player who is highly organised with excellent time management and record keeping
• Experience in carrying out clinical and translational research, working in the field of immune mediated inflammatory diseases
• Knowledge of best practices in primary human cell culture
• Experience in flow cytometry and other assays of immune function, including cell-based assays and gene expression assays
• A keen willingness to learn and evolve skills and knowledge
• Good IT skills with knowledge of databases and processes relating to biobanking and archiving of human biological material
• Ability to work flexible hours including occasional evenings and weekends, when required
1. BSc or MSc in relevant discipline
2. Excellent communication skills
3. Project management in clinical or translational research
4. Experience in human primary cell culture (immune cells would be advantageous)
5. Experience in flow cytometry and other assays of immune function, including cell-based assays
6. Knowledge of databases and processes relating to biobanking and archiving of human biological material
7. Enthusiastic and adaptable team player
1. Knowledge of the immunological basis of immune mediated inflammatory diseases
2. Experience in data analysis (primarily Flowjo but experience in R would be advantageous)
3. Good IT skills
For further information about the post or the project please contact Prof Andrew Cope at firstname.lastname@example.org