A postdoctoral fellow position is available in the laboratory of Robert Köchl in the Department of Immunobiology at King’s College London.
The Köchl lab together with ImmTune Therapies are interested in developing improved methods of generating CAR T cells. In this project we will transfect T cells with chimeric antigen receptor constructs and then characterize both the phenotype of these cells as well as their ability to lyse target cells in vitro and in vivo.
Talented and motivated applicants passionate about doing research at the interface of biotech and academia are invited to apply for this postdoctoral position. The position is initially for a year.
This post will be offered on an a fixed-term contract for 12 months
This is a full-time post - 100% full time equivalent
• Work with mice
• Isolate immune cells from mouse tissues
• Culture and transfect cells either on their own or in co-culture systems
• Analyze cells by flow cytometry
• Carry out histology on tissue sections from mice
The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.
Skills, knowledge, and experience
1. Undergraduate degree in Biological/Biomedical Sciences or related subject
2. PhD awarded in Immunology, Cell Biology, Molecular Biology or related subjects
3. Extensive experience in purifying T and B cells from mouse organs or blood
4. Extensive experience in viral transduction methods for T cells to overexpress genes and to make CAR T cells
5. Experience of working with primary human immune cells.
6. Extensive experience and knowledge about phenothying T cell subsets as well as other immune cell types
7. Multi-colour flow cytometric staining and analysis
8. Holder of a Home Office Personal License
9. Experience with Microscopy
10. Experience with Biochemistry experiments, such as immuno blotting