We are inviting researchers to join an exciting project aiming to solve the mechanisms regulating TDP-43 expression level in mouse and human knockin models of ALS-FTD. Centred at the Denmark Hill at King’s College London, the research assistant will work closely with postdoctoral scientists to perform proteomic studies, CRISPR/Cas9 screening in human cell lines including stem-cell-derived neurons, and spatial transcriptomic studies (Visium, pciSeq, single cell seq and Nanostring) in mouse brain. An interest in working with animal models is important. These projects will involve occasional visits to collaborator’s laboratories in Warwick, the Sanger Institute and Paris.
Day to day roles will include the maintenance of lines of HEK cells and human induced pluripotent stem cells (iPSC), and a range of basic lab duties such as ensuring buffers are kept in stock and fridges/freezer inventories are kept up to date. There will be excellent opportunities for you to develop your own independent projects and you will be an author on any projects to which you make a substantial contribution.
This post will be offered on a fixed-term contract to 05/10/26
This is a full-time post - 100% full time equivalent
• Maintaining cell lines
• Assisting postdocs with basic molecular/biochemical/cellular studies e.g. immunoblotting, PCR, qPCR, immunostaining, confocal microscopy
• Assisting postdocs with mouse studies e.g. Genotyping, setting up behavioural assays, harvesting tissues and processing for immunohistochemistry/transcriptomic studies.
• Assisting postdocs with data analysis e.g. cell and tissue image analysis, mouse behavioural data analysis
• General laboratory maintenance e.g. maintaining cell lines, making up buffers.
• Processing samples at collaborators’ laboratories
The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.
Skills, knowledge, and experience
• Degree in Bioscience
• Knowledge of genetics
• Knowledge of neuroscience and passion for neurodegeneration research
• Good communicator both verbally and written in English
• Good team player and happy to assist co-workers in the lab
• Able to work independently where needed
• Basic lab skills: DNA/protein isolation, PCR, western transfer, buffer preparation, immunocytochemistry
• Scientific publications as a contributing author
The post will be available in October 2021 at the earliest
This post is subject to Disclosure and Barring Service and Occupational Health clearance.
TDP-43, ALS, CRISPR