Research Associate in Centre for Craniofacial and Regenerative Biology
A postdoctoral research associate position is available in the Knight group to work on an exciting project investigating the importance of the chromatin modifying enzyme Ezh2 during muscle regeneration. Candidates with a strong background in zebrafish genetics, molecular biology and imaging are sought who can bring this project to a successful conclusion.
Using live cell imaging, gene manipulation and measures of gene expression the project aims to identify global cues regulating effective muscle regeneration with a focus on epigenetic signals. The project has identified a novel Ezh2-dependent change to a metabolic pathway concomitant with changes to muscle stem cell and immune cell responses to injury. The goal is to now confirm the role of this metabolic pathway in muscle regeneration by manipulation of gene function in zebrafish larvae.
The Knight group utilise live cell imaging and molecular profiling to define the key regulators of muscle homeostasis and repair in the context of disease and ageing. A major focus of the group is to combine descriptors of cell behaviour with molecular readouts for establishing mathematical models of tissue regeneration.
The Centre for Craniofacial and Regenerative Biology combines strengths in tissue engineering and applying animal models for understanding human disease and regeneration. The post holder will be integrated into a welcoming and friendly environment with strong links to other departments at the Guy's campus.
This post will be offered on an a fixed-term contract for 5 months.
This is a full-time post - 100% full time equivalent.
- Perform experiments at the direction of the line manager and at their own initiative to advance the goals of the project
- To record all results and data generated in the course of the work and make them available to the line manager to the agreed standard of the group.
- To take responsibility of and proactively manage zebrafish lines as agreed with the line manager in compliance with all local and national regulations and rules.
- To contribute to the production of research reports, publications and communications as requested and contribute to group meetings.
- To work in a timely and organised fashion to complete pieces of work, produce reports or analyses when requested and contribute to meetings to a required standard.
- To work to an appropriate level of health and safety and perform such jobs as required for effective running of the laboratory.
The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.
Skills, knowledge, and experience
1. PhD awarded in a relevant subject (or thesis submitted)
2. Experience of molecular cloning and gene manipulation
3. Experience of using zebrafish as an experimental animal
4. Experience of using immunolabelling and gene expression analysis using qPCR
5. Experienced user of confocal and light microscopy
6. Excellent practical laboratory skills
7. Excellent computing skills and data management experience
8. Rigorous and critical approach to data acquisition and recording
9. Competent in statistical analysis methods
10. Friendly, helpful and flexible attitude to team working
1. Experience of using CRISPR-Cas9 for gene knockdown in zebrafish
2. Experience of live imaging and image analysis
3. Good grounding in field of tissue regeneration
*Please note that this is a PhD level role but candidates who have submitted their thesis and are awaiting award of their PhDs will be considered. In these circumstances the appointment will be made at Grade 5, spine point 30 with the title of Research Assistant. Upon confirmation of the award of the PhD, the job title will become Research Associate and the salary will increase to Grade 6.
Interviews will be held mid-April with a view to appoint to the post as soon as possible.
Interviews will be held virtually and applicants will be asked to present a short summary of their work to the panel.
This post is subject to Disclosure and Barring Service and Occupational Health clearance.