Research Associate 2
Position available in The Schalper Research Lab in the Department of Pathology at Yale University. The Schalper lab is focused on understanding the immunobiology of human solid tumors using quantitative pathology, computational image analysis, in vitro cell models and molecular biology to interrogate relevant immunomodulatory pathways and identify determinants of sensitivity and resistance to treatments. The candidate will be responsible for preparing in vitro culture samples from blood and tissue specimens for in vitro experimental analysis. Activities include also the study of tissue-based biomarkers in human tissue samples from retrospective cohorts and/or clinical trials using multiplexed quantitative imaging platforms. Data analysis include comparisons of continuous variables, linear functions and survival statistics including uni- and multivariate methods. Sample collection and storage as well as ordering of wet lab and imaging reagent stocks, and other specialized routine procedures are also expected.
Researches and collects data through complex laboratory/ scientific experiments, techniques, and procedures, library research, structured interviews, or through other means for designated research assignments. Records and compiles information related to research data. Codes data accordingly to research specifications. Uses a computer terminal to input and retrieve data and to generate reports. Processes and summarizes data using scientific or statistical techniques. Assists in data interpretation and analyses. Sets up, operates and maintains laboratory equipment and apparatus. Orders and maintains inventory of supplies. May assist in developing, and modifying research experiments, procedures. Performs additional functions incidental to research activities, including collection and processing of samples from clinical trials in immune-oncology.
1. Designs, plans, implements and evaluates complex multifaceted research projects/systems, including method development, adaptation and validation. 2. Collaborates with PI to define research endeavors and the development of research hypothesis and approach. 3. Solves complex methodology, protocol, procedural and research problems through design of techniques, procedures and policies that will achieve research goals. 4. Develops long range plans for supplies and equipment to ensure smooth operation of laboratory. 5. Investigates, analyzes and evaluates complex data, data collection systems and methods to reach scientific conclusions and ensures the integrity of research data. 6. Collaborates with PI to coordinate major lab renovations working with Facilities, Health and Safety, and Purchasing to ensure deadlines, safety standards and purchases are according to plans and within budgets. 7. Prepares scientific reports and papers for research proposals and published reports. 8. May perform other duties as assigned.
Required Education and Experience
Master’s Degree in a scientific discipline and three years of experience or an equivalent combination of education and experience.
Required Skill/Ability 1:
Previous experience with tissue culture using multiple cell lines and emphasis in immune cells. Specimen and/or tissue processing including PBMC, extraction of immune cell subsets using Ficoll gradients and germline DNA. Expertise collecting and processing samples from immune-oncology clinical trials and preparation of samples for cryopreservation and single cell analysis.
Required Skill/Ability 2:
Ability to do quantitative and multiplex immunofluorescent staining in formalin-fixed paraffin embedded tissue samples. Advanced proficiency with statistical software and survival analysis using specialized software (specifically Excel, GraphPad, X-Tile and JMP). Advanced proficiency with quantitative pathology software including AQUAanalysis, InForm and QUpath.
Required Skill/Ability 3:
Excellent verbal and written communication skills. Ability to effectively convey ideas and contribute to reports. Ability to read, understand and interpret documents, protocols, SOPs, safety standards, maintenance instructions and procedure manuals for laboratory equipment.
Required Skill/Ability 4:
Ability to operate instruments and scientific equipment.
Required Skill/Ability 5:
Ability to do the operation of Vectra Polaris and PM2000 AQUA microscopes and associated software as well as the operation of Nanostring nCounter Sprint and nSolver system as well as expertise in managing data from Imaging Mas Cytometry.
Preferred Education, Experience and Skills:
A degree in life sciences and at least two years of research laboratory experience or an equivalent combination of education and experience in life sciences.
Weekend Hours Required?
Evening Hours Required?
Background Check Requirements
All candidates for employment will be subject to pre-employment background screening for this position, which may include motor vehicle, DOT certification, drug testing and credit checks based on the position description and job requirements. All offers are contingent upon the successful completion of the background check. Please visit www.yale.edu/hronline/careers/screening/faqs.html for additional information on the background check requirements and process.
The intent of this job description is to provide a representative summary of the essential functions that will be required of the position and should not be construed as a declaration of specific duties and responsibilities of the particular position. Employees will be assigned specific job-related duties through their hiring departments.
Affirmative Action Statement:
Yale University considers applicants for employment without regard to, and does not discriminate on the basis of, an individual’s sex, race, color, religion, age, disability, status as a veteran, or national or ethnic origin; nor does Yale discriminate on the basis of sexual orientation or gender identity or expression. Title IX of the Education Amendments of 1972 protects people from sex discrimination in educational programs and activities at institutions that receive federal financial assistance. Questions regarding Title IX may be referred to the University’s Title IX Coordinator, at TitleIX@yale.edu, or to the U.S. Department of Education, Office for Civil Rights, 8th Floor, Five Post Office Square, Boston MA 02109-3921. Telephone: 617.289.0111, Fax: 617.289.0150, TDD: 800.877.8339, or Email: email@example.com.
Yale University is a tobacco-free campus