Research Specialist

California, United States
03 Jan 2021
End of advertisement period
03 Mar 2021
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Full Time

Caltech is a world-renowned science and engineering institute that marshals some of the world's brightest minds and most innovative tools to address fundamental scientific questions. We thrive on finding and cultivating talented people who are passionate about what they do. Join us and be a part of the diverse Caltech community.

Job Summary

Studies of small molecule DNA repair inhibitors for use in chemotherapy.  This project involves hit-to-lead development of a new class of DNA repair inhibitors.  Novel chemical compounds will be screened biochemically for potency and specificity and physiologically for synthetic lethality with various tumor  models and on target functions.  This will constitute a unique training opportunity for introduction into the biotechnology industry.

Job Duties

  • DNA preparation, Primer design, Cloning, PCR, RT-PCR
  • Human tissue cell culture-mycoplasma monitoring, maintining cell line collection, freezing, thawing, cataloging cell lines
  • Transfection and siRNA and shRNA knockdowns
  • Cell viability assays
  • Biochemical assays of enzymes that function in DNA repair or replication
  • Protein expression and purification
  • Data analysis, statistical analysis
  • Use of NCBI, PubMed, BLAST, Genbank;  Protein Data Base
  • Project management: Planning, record keeping,
  • Lab management and ordering

Basic Qualifications

  • B.S. degree and at least 4 years of experience in quantitative molecular biology and cell biology in an academic research lab or in the pharmaceutical industry
  • Demonstrated ability to work independently in execution of experiments, analysis of results, and design of follow-up experiments
  • Experience in project management
  • General techniques:  Handling DNA and RNA, protein and nucleic acid electrophoresis; Western blotting; Immunoprecipitation, extract preparation, quantitative enzyme assays (nuclease, polymerase, helicase, topoisomerase or other kinetic analyses),  transfection and transformation; light mcroscopy
  • Special techniques (previous experience appreciated but training provided where required):  site-directed mutagenesis, Immunofluorescence, immunoprecipitation, affinity purification, protein exression and purification, cell vibility assays;  techniques for monitoring DNA replication and recombination, non-B DNA structures , and telomeres.
  • Familiarity with spectrophotometer, scinitillation counter, phosphorimager (Image J, Imagequant), FPLC, plate readers. Gel doc systems, Licor imaging
  • Fluency in Word, Excel, GraphPad Prism, Powerpoint

Preferred Qualifications

  • PhD or PhD level experience in academia or in the biotechnology industry
  • A subset of the skills listed above
  • Background knowledge in the field of DNA repair, replication or recombination appreciated but not required
  • Background knowledge in cancer research preferred but not required
  • Ability to teach techniques to others

Required Documents

  • Resume with publication list
  • Transcript (undergraduate and graduate)

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