Life Sciences Technician II
Stanford University seeks a Life Science Technician II (LST II) at 100% effort for a 1 year term (subject to possible renewal) to join the Ocean Conservation Genomics team at the Hopkins Marine Station in Pacific Grove, CA. The focus of the Ocean Conservation Genomics team is to use advanced DNA sequencing approaches to uncover the genetic basis of traits important to survival for marine life in the future, to monitor species genetic diversity, and to generate genomic tools that allow more powerful interventions into ocean ecosystem resilience. A successful future member of the team will be able to curate and manage the large data sets generated by modern genomic sequencing, be able to sift these data sets for patterns, and be able to work with the other team members to devise and test hypotheses based on these data. Some laboratory work in basic DNA manipulations (extraction, PCR, library construction, sequencing preparation) will be needed periodically. This person will also be able to work collaboratively, follow deadlines, suggest new approaches, support the work of others on the team, and overall contribute to a sense of community within the team.
The duties listed under this section are designed to provide a representative sampling of key tasks and/or responsibilities associated with the job. They are not intended to be a complete list of all the duties performed by employees in the classification.
Characteristic duties include:
- Perform basic lab extractions, amplifications, library preparations, etc for genetic data collection.
- Curate and maintain computer data sets from field and genome work.
- Use of software tools to collate and manipulate genome data sets.
- Conduct statistical analyses of patterns
- Display data patterns and test hypotheses generated in weekly conference calls.
- Maintain a running log of data sets, patterns and analyses performed
- Perform animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals.
- Immunohistological procedures including frozen sectioning and multi-step immunologic staining for fluorescence or enzymatic detection. Some level of histologic assessment may be required.
- Maintenance, transfer and establishment of cell lines from normal or tumor cells, preparation and bioassay of serum, screening for contamination, isolation of cell mutants, cell staining, mass production of cells and cell cloning.
- Fixation, dehydration, staining and embedding of specimens for electron microscopy; carbon coating, negative staining, shadowing, operation of electron microscope (EM) either transmission or scanning; use of an ultramicrotome with a diamond knife for routine thin sectioning; alignment of an EM where the system is automated.
- Purification and /or structural characterization of compounds for which a protocolis well established.
- Separation of cells, organelles, or molecules using density gradients.
- Radioimmunoassay of viruses, proteins, or hormone levels in cell extract according to established protocol.
- Techniques such as electrophoresis, chromatography, autoradiography, radiolabeling of animals, cell cultures, or macromolecules.
- Isolation, purification and maintenance of mammalian viruses and bacteriophages; determination of viral concentration.
- Genetic manipulation of bacteria including mutagenesis, ‘phage transduction and transformation.
- Accurate documentation of experimental protocols and basic statistical analysis of data.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.
- Ability to stand and move on hard surfaces for up to eight hours.
- Occasionally lift and handle materials up to 10 pounds.
- Rarely carry/pull and/or push up to 20 pounds.
- Must have correctible vision to perform duties of the job.
- Position may require repetitive motion.
Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required.
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University’s Administrative Guide, http://adminguide.stanford.edu/. and the appropriate collective bargaining agreement at https://cardinalatwork.stanford.edu/hr processes policies/labor-relations-collective bargaining
- Schedule: Full-time
- Job Code: 4440
- Employee Status: Fixed-Term
- Grade: G
- Department URL: http://urology.stanford.edu/
- Requisition ID: 88095