Life Science Research Professional 1
The Lewis Lab in the Department of Molecular and Cellular Physiology (School of Medicine) studies the mechanisms and consequences of signaling by store-operated calcium channels. This class of ion channels forms a major pathway for calcium entry in almost all cells and is particularly critical in the immune system, where they are required for the activation of T lymphocytes during the immune response. The lab seeks to understand how these channels are regulated at the cellular and molecular level, and how their activity controls essential cellular processes (gene activation, motility, secretion, and immunological synapse formation).
The Lewis Lab is seeking a Life Science Research Professional 1 to provide essential support for several projects. Specific activities will include: CRISPR/Cas9-mediated gene tagging and deletion in cultured cell lines, generation and maintenance of stable mutant cell lines, site-directed mutagenesis and subcloning of DNA constructs, standard biochemical assays including western blots, and preparing and applying expression constructs for purification of proteins from bacteria and insect cells. The LSRP will also carry out experiments to examine the effects of mutant proteins on cell functions, as well as oversee lab operations and train new lab personnel in molecular biology techniques as needed.
- Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
- Independently conduct experiments; maintain detailed records of experiments and outcomes.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
- Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
- Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assigned
DESIRED QUALIFICATIONS :
Bachelor’s degree in molecular biology or related field.
Expertise in CRISPR/Cas9-mediated gene editing.
Experience with standard molecular biology techniques including PCR, site-directed mutagenesis, subcloning; SDS-PAGE and western blots.
Experience with mammalian cell culture and single-cell cloning.
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University
- Schedule: Full-time
- Job Code: 4945
- Employee Status: Regular
- Grade: E
- Requisition ID: 87254