Trainee, Research Officer, SCELSE
In conjunction with Workforce Singapore, NTU offer traineeships to support the SGUnited Traineeships Programme. The Traineeships are part of NTU's efforts to create relevant and meaningful traineeship opportunities for our nation’s new graduates entering the workforce during this difficult time.
About the Singapore Centre for Environmental Life Sciences Engineering (SCELSE)
The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Singapore Ministry of Education, Nanyang Technological University and National University of Singapore. Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from the emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.
The Kline lab at SCELSE is looking for a motivated and detail-oriented individual to work on advanced in vitro models to study host-pathogen interactions in infective endocarditis caused by Enterococcus faecalis. The trainee will work together with Dr. Haris Antypas under the supervision of Assoc. Prof. Kimberly Kline. S/he will be primarily trained in microfluidics, live imaging, image analysis, and molecular methods for gene expression analysis.
The main task of this position will be to assemble and use a microfluidic model that mimics the heart valve environment and study the effect of fluid flow on i) bacterial adhesion, ii) bacterial transcriptome and iii) neutrophil-bacteria interactions. The trainee will perform time-lapse microscopy to monitor how bacteria behave on different surfaces under fluid flow and subsequently quantify adhesion by video analysis using image. S/he will also isolate RNA from bacteria exposed to fluid flow to investigate gene expression with PCR. Finally, s/he will also introduce neutrophils in this microfluidic model to investigate the release of neutrophils extracellular traps against E. faecalis. If time permits, s/he will also be trained in experimental animal handling and be introduced to microsurgical techniques to assist in in vivo experiments for infective endocarditis. The trainee is also expected to keep an organized lab book and communicate his/her findings efficiently on our weekly lab meetings.
- Laboratory experience in basic microbiology, microscopy, molecular biology, and immunology techniques
- Demonstrable experience in research documentation and communication of research findings
- Experience in experimental animal handling
Hiring Institution: NTU