Electron Microscopy Research Associate

Connecticut, United States
29 Jun 2020
End of advertisement period
29 Aug 2020
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Fixed Term
Full Time

Position Focus:

Under the direction of the Principal Investigator and working in collaboration with laboratory personnel, this position will be in charge of the design, collection, optimization and interpretation of electron microscopy research in the Colón-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. Work includes preparation of scientific reports and papers for research proposals and published records. The position will prepare and process C. elegans worms for electron microscopy to better understand the function and structure of the C. elegans nervous system. Specific duties include but are not limited to:

  • Review literature and data generated in the lab in order to recommend the design of new experiments and protocols to address the research questions laid out by the PI. Recommend new research questions based on findings.
  • Analyze, develop, and interpret all the data collected through EM techniques.
  • Work with lab members and extramural collaborators to coordinate projects and approaches.
  • Prepare electron microscopy data for presentations, including published scientific reports and seminars.
  • Sample preparation techniques, including:
    • Fix C. elegans larvae and embryos by high pressure freeze and freeze substitution (HPF/FS).
    • Embed, trim, and orient fixed samples.  Cut and stain serial sections at specific ROI.
    • Prepare samples for immunolabeling and perform immunogold staining.
  • Process electron micrographs, including montaging, alignment, segmentation, and 3D rendering.
  • Perform advanced microscopy methods such as electron tomography, Focus-Ion-Beam SEM (FIB-SEM) 3D volume imaging as needed by projects.

Essential Duties

  1. Recommends the design and implementation of procedures and protocols under the direction of a higher-level authority.
  2. Investigates, analyzes, and reaches preliminary scientific conclusions related to research planning
  3. Ensures data results are compiled, analyzed, and presented properly.
  4. Analyzes, develops and interprets scientific results. Maintains clear documentation of results. Prepares scientific reports and papers for research proposals and published reports.
  5. Performs independent research and laboratory techniques and participates in experimental research planning.
  6. Interacts with internal contacts including the PI, research assistants, graduate students, Radiation Safety and Biological Safety officers, and Animal Care personnel to discuss and evaluate research results and to ensure the smooth and safe operation of the laboratory.
  7. Interacts with external contacts such as researchers at other institutions, staff at biotechnology and reagents companies to consult on research methods and to keep abreast of product information and developments. 8. May perform other duties as assigned.

Required Education and Experience

Master’s Degree in a scientific discipline and one year experience or an equivalent combination of education and experience.

Required Skill/Ability 1:

Ability to coordinate laboratory projects, establish and maintain effective working relationships with supervisors, collaborators and other employees.

Required Skill/Ability 2:

Well-developed understanding of procedures and techniques used in Electron Microscopy with the ability to adapt to new procedures concerning work in electron microscopy of C elegans.

Required Skill/Ability 3:

Ability to perform tasks requiring a high degree of attention to detail.

Required Skill/Ability 4:

Well-developed verbal communication skills.

Preferred Education, Experience and Skills:

Master's Degree in biology or Neuroscience and one year experience. At least 3 years of experience in biological electron microscopy. Desired expertise in immuno electron microscopy, FIB-SEM and electron tomography (room temperature) for biological sciences. Knowledge of Cell Biology and Neuroscience and experience working with teams.

Weekend Hours Required?


Evening Hours Required?


Drug Screen


Health Screening


Background Check Requirements

All candidates for employment will be subject to pre-employment background screening for this position, which may include motor vehicle, DOT certification, drug testing and credit checks based on the position description and job requirements. All offers are contingent upon the successful completion of the background check. Please visit www.yale.edu/hronline/careers/screening/faqs.html for additional information on the background check requirements and process.

Posting Disclaimer

The intent of this job description is to provide a representative summary of the essential functions that will be required of the position and should not be construed as a declaration of specific duties and responsibilities of the particular position. Employees will be assigned specific job-related duties through their hiring departments.

Affirmative Action Statement:

Yale University considers applicants for employment without regard to, and does not discriminate on the basis of, an individual’s sex, race, color, religion, age, disability, status as a veteran, or national or ethnic origin; nor does Yale discriminate on the basis of sexual orientation or gender identity or expression. Title IX of the Education Amendments of 1972 protects people from sex discrimination in educational programs and activities at institutions that receive federal financial assistance. Questions regarding Title IX may be referred to the University’s Title IX Coordinator, at TitleIX@yale.edu, or to the U.S. Department of Education, Office for Civil Rights, 8th Floor, Five Post Office Square, Boston MA 02109-3921. Telephone: 617.289.0111, Fax: 617.289.0150, TDD: 800.877.8339, or Email: ocr.boston@ed.gov.


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