Senior Research Support Associate, Sharp Lab

3 days left

Location
Massachusetts, United States
Posted
15 Jun 2020
End of advertisement period
15 Aug 2020
Ref
18764
Contract Type
Permanent
Hours
Full Time

Working at MIT offers opportunities, an environment, a culture – and benefits – that just aren’t found together anywhere else. If you’re curious, motivated, want to be part of a unique community, and help shape the future – then take a look at this opportunity.

RESEARCH SUPPORT ASSOCIATEKoch Institute for Integrative Cancer Research, to provide comprehensive technical support for an ongoing single-cell technology development project under the supervision of a postdoctoral associate in the laboratory of Philip A. Sharp. Existing single-cell sequencing methods focus on protein-coding genes, but overlook other categories of RNA, which play critical role in cellular structures and functions. Understanding the regulation of non-coding RNA in single-cells can provide insights on fundamentals of gene regulation in health and disease. This technology development project sits at the interface of molecular biology, biochemistry, and chemistry.  Will assist in a wide array of experiments to develop and optimize the new methodology. Responsibilities include executing and optimizing ongoing experiments; analyzing results and designing follow-up experiments based on the results; presenting findings and insights at group meetings; recording and organizing data generated from the experiments in a clear, concise, and accessible format; culturing cells of murine embryonic stem cells and various cancer cell lines; and processing cell materials for preparation of RNA sequencing libraries and preparations of next-generation sequencing libraries.

Job Requirements

REQUIRED high school diploma or its equivalent; knowledge of general laboratory practices and procedures; experience with lab techniques, including cloning (including TOPO and restriction cloning), western blotting, PAGE, reverse transcription, and quantitative PCR; next-generation sequencing experience, either in an academic institution or industrial setting; strong problem-solving and organizational skills; ability to work independently and as part of a team; self-motivation; and a commitment to producing high-quality work.  

PREFERRED bachelor’s degree; basic understanding of Illumina sequencing technology, fluorescent microscopy, and flow cytometry; and familiarity with computational languages such as R and Python. Job #18764-4

MIT is an equal employment opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, sex, sexual orientation, gender identity, religion, disability, age, genetic information, veteran status, ancestry, or national or ethnic origin.

MIT considers equivalent combinations of experience and education for certain jobs. All candidates who believe they possess equivalent experience and education are encouraged to apply.

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