Life Science Technician II

Location
California, United States
Posted
10 Jun 2020
End of advertisement period
10 Aug 2020
Ref
86730
Academic Discipline
Life sciences, Veterinary Science
Contract Type
Permanent
Hours
Full Time

The Czechowicz Lab within the Division of Stem Cell Transplantation and Regenerative Medicine, in the Department of Pediatrics at Stanford University School of Medicine is seeking a motivated, friendly and well-organized Life Science Technician II to perform basic functions and activities involved in exciting research projects. This a dynamic and stimulating place to work, focused on excellence in research, education and patient care. The Czechowicz lab is focused on hematopoietic stem cell biology and further exploring how hematopoietic stem cells interact with their microenvironment through basic science and translational medicine particularly in bone marrow failure to ultimately improve outcomes for these patients and many others.

Our laboratory has previously conducted pioneering work demonstrating that hematopoietic stem cell (HSC) depletion is a critical component to donor HSC engraftment. We have developed multiple pre-clinical agents that enable safe and targeted HSC conditioning, several of which are turning into promising clinical therapies. We are now using these agents as probes to understand underlying hematopoietic biology and are applying these to the treatment of candidate diseases of great need. We are primarily focused on blood and immune diseases, but this work could also potentially be expanded to other organ systems as well.

The lab strives to maintain a highly collaborative, dynamic and positive environment. The Life Science Technician II would be a great addition to the team and help with exciting basic and translational experiments that hopefully will result in exciting new therapies for patients.

Duties include:

  • Assist research team with animal (mouse)-based experiments. Plan and adhere to a timeline for animal experiments. Perform basic procedures such as peripheral bleeds and injections and prepare samples for further testing. Assist with FACS analysis of samples and subsequent data analysis for presentation to PI and lab peers as scheduled. They will be the key manipulator in the technical set-up and check-points of mouse in vivo experiments
  • Keep updated with the literature related to the project.
  • Work with veterinary team to ensure methods and procedures are optimal for health and safety of animals and share them with other members of the lab. Ensure that animal protocols are updated with all planned experimentation and that these are followed by laboratory team.
  • Keep track of all experimentation in laboratory notebook and keep careful records of all animal use and animal breeding. Discuss this with lab manager and PI to ensure proper use and budgeting.
  • Coordinate with lab members regarding general lab maintenance, supply inventory, and safety.
  • Check animal cages at least 3 times per week. Set-up timed pregnancies for animals as required. Liaise with veterinary staff concerning care of experimental animals. Affix “pink cards” to animal cages to indicate special care concerning weaning and nutrition. Follow-up morbidity reports with veterinary staff.
  • Check animal cages at least 3 times per week. Set-up timed pregnancies for animals as required. Liaise with veterinary staff concerning care of experimental animals. Affix “pink cards” to animal cages to indicate special care concerning weaning and nutrition. Follow-up morbidity reports with veterinary staff. Alert veterinary staff of abnormal animal physical presentation/behavior. At sacrifice, sample tissues and organs so that they are suitable for immunohistochemistry analysis and/or isolated cells according to the experimental needs defined with the PI.
  • Assist research team with animal (mouse)-based experiments. Plan and adhere to a timeline for animal experiments. Perform basic procedures such as peripheral bleeds and injections and prepare samples for further testing. Assist with FACS analysis of samples and subsequent data analysis for presentation to PI and lab peers as scheduled. They will be the key manipulator in the technical set-up and check-points of mouse in vivo experiments
  • Keep updated with the literature related to the project.
  • Work with veterinary team to ensure methods and procedures are optimal for health and safety of animals and share them with other members of the lab. Ensure that animal protocols are updated with all planned experimentation and that these are followed by laboratory team.
  • Keep track of all experimentation in laboratory notebook and keep careful records of all animal use and animal breeding. Discuss this with lab manager and PI to ensure proper use and budgeting.
  • Coordinate with lab members regarding general lab maintenance, supply inventory, and safety.
  • Check animal cages at least 3 times per week. Set-up timed pregnancies for animals as required. Liaise with veterinary staff concerning care of experimental animals. Affix “pink cards” to animal cages to indicate special care concerning weaning and nutrition. Follow-up morbidity reports with veterinary staff.
  • Check animal cages at least 3 times per week. Set-up timed pregnancies for animals as required. Liaise with veterinary staff concerning care of experimental animals. Affix “pink cards” to animal cages to indicate special care concerning weaning and nutrition. Follow-up morbidity reports with veterinary staff. Alert veterinary staff of abnormal animal physical presentation/behavior. At sacrifice, sample tissues and organs so that they are suitable for immunohistochemistry analysis and/or isolated cells according to the experimental needs defined with the PI.

Additional Information

  • Schedule: Full-time
  • Job Code: 5644
  • Employee Status: Regular
  • Grade: A24
  • Department URL: http://pediatrics.stanford.edu/
  • Requisition ID: 86730

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