Life Science Research Professional
FTE: 100% FTE
The Weinacht lab at Stanford School of Medicine is seeking a highly motivated, collaborative and well organized Life Science Research Professional with interest in the stem cell biology, hematopoiesis, immunology and metabolism. Successful candidates will join the Department of Pediatrics, Division of Stem Cell Transplantation and Regenerative Medicine at Stanford School of Medicine.
Our laboratory is interested in understanding the fundamental mechanisms that link hematopoietic stem cell fate/differentiation to metabolism. To do so, we study single gene mutations affecting hematopoietic stem and progenitor cell development that lead to severe human disease. We have engineered an advanced cellular model systems by CRISPR-Cas9 gene editing to model disease-causing immune and hematopoietic defects in primary human hematopoietic stem cells. Reticular dysgenesis (RD) is one of the most serious forms of severe combined immunodeficiency (SCID). Patients with RD suffer from a combined block in neutrophil and lymphocyte development. The disease is caused by defects in the mitochondrial enzyme Adenylate Kinase 2 (AK2). How defects in AK2 translate into the disease phenotype has long remained elusive. The LSRA will be a member of the research team that is dissecting the mechanistic basis of this fascinating disease on a cellular and molecular level to help elucidate the intricate interplay between cellular metabolism, oxidative stress and cell death in a state of health and disease. The LSRA will generate and characterize CRISPR-edited stem and progenitor cells, acquire expertise in targeted differentiation techniques and develop/refine his/her repertoire of basic and advanced molecular, biochemical and cell biological techniques in order to investigate questions of metabolism, proliferation, differentiation and apoptosis. Studies may involve drugs, recombinant DNA, stem cells and murine models.
Our laboratory works in close collaboration with other laboratories on campus, particular in the Stem Cell Institute and the Bioengineering Department at Stanford University and beyond. Candidates with outstanding interpersonal competencies, intrinsic motivation and skills in molecular biology/cell biology/biochemistry/bioinformatics or related fields are encouraged to apply. The PI has a particular interest in cultivating the careers of aspiring scientists to help them achieve their long-term professional goals. The candidate can expect individualized mentorship tailored to their personal career goals and a positive and goal-oriented work environment. The position can be adapted for an individual who is seeking to further methodical expertise and CV building before pursuing a competitive higher academic graduate education or for an experienced individual who is ultimately seeking an academic research scientist or scientific managerial position. Candidates must adhere to highest ethical principles when working with laboratory animals.
• Independently conduct a portion of the research project(s). Make recommendations on experimental design and/or research direction to principal investigator.
• Independently conduct complex (lab and/or field) experiments to gather data for a portion of a major research project.
• Interpret and analyze results, often where few guidelines exist, and make modifications to existing procedures and protocols.
• Develop new research protocols where appropriate protocols are not described in the literature or where modification or adaptation of standard procedures and protocols is required.
• Perform ongoing literature review to remain current with new procedures and related research; apply to ongoing research and development of new protocols.
• Contribute substantially to the preparation of papers for publication, especially in the results section, and to publication of findings. Present ongoing work to colleagues and/or academic conferences.
• Work with health and safety department to ensure lab complies with regulations and all required trainings.
• Supervise (either formally or informally) staff or students as needed, including oversight and direction on techniques, as well as consultation on project work. Serve as a technical resource for other research staff.
• Oversee the daily activities of the laboratory including lab maintenance; maintain lab stock, manage chemical inventory and safety records, and provide general managerial lab support as needed. May assist with purchasing laboratory equipment.
*- Other duties may also be assigned
• Master’s degree or PhD in Biology, Biochemistry, Chemistry, Molecular biology or other related life-science discipline
• Proficiency in scientific writing in English
• Exceptional attention to detail and meticulous execution and record of experiments is essential
• Must be able to think critically and is encouraged to design experiments with guidance
• Basic understanding of biological principles
• Strong communication skills
• Must adhere to highest ethical principles when working with laboratory animals
EDUCATION & EXPERIENCE (REQUIRED): Master’s degree in related scientific field and two years of relevant work experience
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
• Comprehensive understanding of scientific principles.
• General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
• Strong analytical skills and excellent judgment.
• Ability to maintain detailed records of experiments and outcomes.
• Ability to work under deadlines with general guidance is essential.
• Excellent organizational skills and demonstrated ability to accurately complete detailed work.
• Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
• Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
• Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
• Must have correctible vision to perform duties of the job.
• Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
• Ability to lift heavy objects weighing up to 50 pounds.
• Ability to work in a dusty, dirty, and odorous environment.
• Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
• May require working in close proximity to blood borne pathogens.
• May require work in an environment where animals are used for teaching and research.
• Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
• Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
• May require extended or unusual work hours based on research requirements and business needs.
Job Code: 4952
Employee Status: Regular
Department URL: http://pediatrics.stanford.edu/
Requisition ID: 83355