Life Science Technician I

Location
Stanford, California
Posted
04 Jun 2019
End of advertisement period
02 Aug 2019
Ref
83352
Contract Type
Fixed Term
Hours
Full Time

The Department of Ophthalmology is seeking a Life Science Technician 1 for a six-month fixed term to participate in projects for neuroprotection of retinal ganglion cells in glaucoma. The lab focuses on developing adeno-associated virus-mediated gene therapy for retina degeneration induced by optic neuropathies.
The minimum requirements for a Life Science Technician 1 level is generally associated with approximately one year’s employment experience in laboratory work or equivalent training (e.g.: one year of college science). Work requires the ability to follow detailed instructions and keep accurate records. The Life Science Technician will be working closely with Principal Investigator, Dr. Yang Hu, Assistant Professor of Ophthalmology.
Characteristic Duties:
Very simple animal dissection such as removing a readily accessible organ, preparation before and assistance during surgery, administration of medication and post-surgery care.
Histological procedures involving fixation of tissue and staining specimens with stains that do not require microscopic control in proceeding from step to step
Maintenance and transfer of established cell lines where the culture conditions are well defined
AAV purification.
Biochemical assays (often colorimetric or spectrophotometric) of compounds or enzymatic activity using well-established procedures where the determination of any settings or adjustments is provided to the technician
Molecular biology assays (polymerase chain reaction, sequencing reactions) where the protocol is well established
Operation of instruments (e.g., spectrophotometer, fraction collector, scintillation counter, PCR machines, light microscope, cell counter) where the settings are simple or prescribed; techniques such as centrifugation (not involving density gradients) or limited black and white photography
Isolation, purification and maintenance of bacteria and basic manipulation of bacterial plasmids using well-established protocols; determination of the concentration of cells
Complete and accurate documentation of the work performed.