Research Specialist A
The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn’s distinctive interdisciplinary approach to scholarship and learning.
Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play.
The University offers a competitive benefits package that includes excellent healthcare and tuition benefits for employees and their families, generous retirement benefits, a wide variety of professional development opportunities, supportive work and family benefits, a wealth of health and wellness programs and resources, and much more.
The University of Pennsylvania School of Veterinary Medicine is a global leader in veterinary education, research, and clinical care. Founded in 1884, the School is a proud member of the One Health initiative, linking human, animal, and environmental health. http://www.vet.upenn.edu/
Molecular biology/Biochemistry: Incumbent will be charged with extensive molecular cloning tasks that include: site directed mutagenesis, CRISPR targeting with gRNA preparation, gene replacement constructs (Cre/loxp), diphtheria toxin-based selection of E/S, lentivirus packaging and recombinant protein engineering. Also, the incumbent must be able to conduct non-radioactive Northern and Southern blotting approaches.
Microscopy: Operation and analysis of confocal microscope and ability to learn how to operate the two-photon microscope.
Lab maintenance: laboratory supplies organization/inventory, standard reagent preparation, ordering and maintenance of a database for replenishment of laboratory supplies.
Cellular immunology/basic microscopy: Basics of aseptic technique, light microscopy, cell culture responsibilities including maintenance of cryopreserved cell lines and stocks.
Basic molecular biology techniques include molecular biology vector cloning, transformation, transfection, southern blotting, nucleic acid extraction, cDNA generation, and quantitative PCR.
Requirements also include a range of basic biochemistry techniques including: ELISA, SDS-PAGE, western blotting, and immunoprecipitation. Also, responsibilities will include histological staining techniques and Immunohistochemistry.
Bachelor’s Degree in the basic sciences and 0-1 year of experience or equivalent combination of education and experience is required.
Experience with basic data entry software, WORD, EXCEL, Image J, Flow jo, and Graphpad Prism and basic knowledge of biochemistry, molecular biology, and Immunology are required. Strong organizational and communication skills are essential. Experience with website design and basic coding experience is desired.
It is essential that the candidate demonstrate excellent communication skills in both written and verbal English.
Penn adheres to a policy that prohibits discrimination on the basis of race, color, sex, sexual orientation, gender identity, religion, creed, national or ethnic origin, citizenship status, age, disability, veteran status, or any other legally protected class.