Department Peter Lukavsky Research Group – Central European Institute of Technology
Deadline 31 Aug 2019
Start date negotiable

A position for a laboratory technician is available in the research group “RNA-based Regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.

Research Group Leader: Peter Josef Lukavsky, Ph.D.

Job Description:

• Expression and purification of proteins
• Transcription and purification of RNAs
• Preparation of various media and buffers
• PCR and cloning of RNA and protein constructs
• Preparation of isotope-labelled nucleotides
• Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis
• Performance of NMR experiments to study small molecule interactions with proteins and RNA
• Implementing new techniques and projects
• Help incoming co-workers into a smooth start in the lab

Requirements:

• At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences
• Good knowledge of English (oral and written)
• Excellent interpersonal and communication skills
• High motivation, flexibility and self-driven interest to learn new things
• Reliability, team player, meticulousness
• Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.
• Computer literacy, MS word, Excel.
• Excellent experience with molecular biology, protein and RNA purification, biochemistry and NMR spectroscopy is a big plus

We offer:

• Interesting work in a top scientific team
• Stable base and excellent instrumental equipment of CEITEC MU
• employment benefits
• Salary based on qualification and experience; part time appointment possible

Brno is an attractive, lively place to live and work with many cultural and recreational amenities. Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references to Peter Lukavsky (peter.lukavsky@ceitec.muni.cz). Candidates considered for the second round of interview will be informed via email.