Laboratory Research Analyst, Anesthesiology
This position will be supported by an NIH-funded Program Project at Duke University titled “Resolution of Neuroinflammation and Persistent Pain by Complementary Approaches.” The overall objective of the Program Project is to investigate the mechanisms whereby complementary and integrative health approaches, such as acupuncture and natural products from fish oil, alleviate pain in pre-clinical models of common chronic pain syndromes. Essential to the Project’s overall objective is the characterization of molecular and cellular markers related to pain, inflammation, and neuroinflammation. Thus, this position will take a lead in the Molecular Profiling Core and be responsible for the planning, development and implementation of a variety of complex tasks, including profiling immune and non-immune cells using flow cytometry and evaluating the expression of proteins related to pain and inflammation using standard mesoscale discovery multiplex assays.
- Conduct a variety of complex and independent investigations in the planning, development and implementation of original experimental procedures and specialized laboratory techniques in the analysis of materials under experimentation.
- Collect information and data through observation, experimentation and various other methods, including the ability to operate highly technical laboratory instruments and equipment.
- Prepare various tissues and specimens for complex procedures, and devise experimental techniques for tests and analyses.
- Evaluate and interpret collected data and prepare reports and analyses in order to set forth progress, identify adverse trends, and provide appropriate recommendations or conclusions.
- Confer with principal investigator in developing plans for research projects and to discuss the interpretation of results and the preparation of scientific manuscripts for publication.
- Review journals, abstracts and scientific literature to keep abreast of new developments and to obtain information regarding previous experiments to aid in the design and development of original procedures and techniques.
- Supervise, train and instruct graduate/undergraduate students, post-doctoral fellows, or lower level personnel in correct laboratory techniques and procedures.
- Perform other related duties incidental to the work described herein.
The above statements describe the general nature and level of work being performed by individuals assigned to this classification.
This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.
Mouse breeding and management (currently 9 distinct strains), genotyping via PCR and being able to establish new genotyping protocols as needed, behavioral phenotyping, survival surgery, and tissue dissection. Immunolabeling techniques (immunohistochemistry, immunofluorescence, western blotting) Immune cell profiling (flow cytometry) Microscopy, confocal microscopy, and light microscopy Molecular biology techniques (q-PCR, ELISAs, cell culture)
Data Management and Analysis: Implement and maintain electronic laboratory notebook (eg, LabArchives) Data analysis in Excel, GraphPad Prism, Qlucore, and/or equivalent programs
Supervisory: Training and management of basic research personnel Oversee daily lab activities Oversee the implementation and optimization of standard operating protocols Ensure all personnel compliant with all federal, state, and institutional requirements
Duke Entity MEDICAL CENTER
Job Family Level 71
Full Time / Part Time FULL TIME
Regular / Temporary Regular
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.
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Essential Physical Job Functions:
Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
Work requires a bachelor's degree in botany, biology, zoology,psychology or other directly related scientific field.
Work requires four years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE