Life Science Technician

Location
California, United States
Posted
11 Jan 2019
End of advertisement period
11 Mar 2019
Ref
81643
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Permanent
Hours
Full Time

The Department of Psychiatry and Behavioral Sciences is seeking a 50% FTE Life Science Technician 1 to participate in projects aiming to dissect the function of non-coding cis-regulatory elements. The lab focuses on studying synaptic function and plasticity using mouse models, primarily making electrophysiological recordings and optogenetic stimulation in brain slices and mouse behavioral studies.

Characteristic Duties:

  • Stereotaxic surgery in mice involving pre- and post-operative surgical procedures and viral injections
  • Histological procedures involving perfusions, fixation of tissue and staining specimens with stains that do not require microscopic control in proceeding from step to step
  • Molecular biology assays (polymerase chain reaction, sequencing reactions) where the protocol is well established
  • Operation of instruments (e.g., PCR machines, light microscope, cell counter) where the settings are simple or prescribed; techniques such as centrifugation (not involving density gradients) or limited black and white photography
  • Isolation, purification and maintenance of bacteria and basic manipulation of bacterial plasmids using well-established protocols; determination of the concentration of cells
  • Maintenance of a mouse colony, including weaning/splitting, breeding and genotyping
  • Design simple experiments based on the experimental design process and adapting standard protocols 
  • Analysis of experimental data and presentation of data in written and oral formats
  • General lab maintenance, including ordering, organization and restocking lab supplies
  • Complete and accurate documentation of the work performed.

The minimum requirements for a Life Science Technician I level is generally associated with approximately one year’s employment experience in laboratory work or equivalent training (e.g.: one year of college science). Work requires the ability to follow detailed instructions and keep accurate records. The Life Science Technician will be working closely with Principal Investigator, Dr. Julie Kauer in the Department of Psychiatry and Behavioral Sciences.