Research Assistant in Molecular Microbiology/Molecular Biology

Sheffield, United Kingdom
Grade 5 - £22,659 to £26,243 dependent on experience (fixed term for 6 months)
08 Dec 2018
End of advertisement period
02 Jan 2019
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Fixed Term
Full Time

Health And Wellbeing

Grade 5 - £22,659 to £26,243 dependent on experience (fixed term for 6 months)

Catheter-associated urinary tract infections (CAUTIs) are amongst the most commonly acquired healthcare associated infections (HCAIs) contributing considerably to patient morbidity and mortality rates, in addition to posing a substantial economic burden to the NHS. With an increasing prevalence of antibiotic resistance in uropathogenic Escherichia coli (UPEC), a leading cause of CAUTI, there has been significant interest in the development of strategies to help prevent infection that avoids the use of antibiotics. One such strategy is to use broad-spectrum biocides as anti-infective catheter coating agents, however the long-term impact of biocide exposure in UPEC is not clearly understood.

We have previously characterised the impact of long-term exposure to a panel of commonly used biocides in a highly virulent, multi-drug resistant UPEC isolate EC958. This has involved evaluating the emergence of resistance, quantifying bacterial biofilm formation and assessing relative pathogenicity in a Galleria Mellonella waxworm model.

The proposed project aims to further understand the phenotypic adaptions observed by unravelling the transcriptomic changes that underpin biocide adaptation in EC958 through the use of RNA-sequencing and qPCR of specific target sites.

Highly motivated, with the ability to work independently and plan and organise effectively, you will also have experience of:

  • Working on research projects
  • Molecular biology, including RNA extraction and gene expression analysis

Experience of analysing next-generation 'omics' data, knowledge of the R programming language and experience of confocal imaging and image analysis would also be advantageous, but aren't essential.

Because of funding restrictions we're looking for the successful candidate to start by 01/02/19 at the latest. If this would be a problem for you please make this clear in your cover letter.

We particularly welcome applicants from underrepresented groups

For non-EU applicants, we won't be able to provide a certificate of sponsorship / visa for this job.

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Contact Person : Dr Sarah Forbes
Contact Number : 0114 225 3075
Date Advertised : 07-Dec-2018
Closing Date : 02-Jan-2019
Employment Type : Fixed Term - Full Time
Location : City Campus