Life Science Technician
The laboratory of Dr. Kristy Red-Horse in the Biology Department is searching for a Life Science Technician 1 to assist in studies investigating the mechanisms of mammalian heart development. The Life Science Technician will be involved in creating and analyzing genetically-engineered mice as well as isolating and processing embryonic cardiovascular tissues for imaging, all with the goal of describing the cellular and molecular processes that lead to heart development. The Life Science Technician’s main role will be to provide experimental support for the various projects in the laboratory Specific duties include mouse colony management and husbandry, histopathology, PCR, immunohistochemistry, varied molecular biology techniques, and lab organization.
The ideal candidate will have previous experience in working with embryonic cardiac tissues, in situ hybridization, confocal microscopy.
- Very simple animal dissection such as removing a readily accessible organ,
- preparation before and assistance during surgery, administration of medicationand post-surgery care
- Histological procedures involving fixation of tissue and staining specimens with stains that do not require microscopic control in proceeding from step to step
- Maintenance and transfer of established cell lines where the culture conditions are welldefined
- Biochemical assays (often colorimetric or spectrophotometric) of compounds or enzymatic activity using well-established procedures where the determination of any settings or adjustments is provided to the technician
- Molecular biology assays (polymerase chain reaction, sequencing reactions) where theprotocol is well established
- Operation of instruments (e.g., spectrophotometer, fraction collector, scintillation counter, PCR machines, light microscope, cell counter) where the settings are simple or prescribed; techniques such as centrifugation (not involving density gradients) or limited black and white photography
- Isolation, purification and maintenance of bacteria and basic manipulation of bacterial plasmids using well-established protocols; determination of the
- concentration of cells
- Complete and accurate documentation of the work performed.
Classification at the Life Science Technician I level is generally associated with approximately one year’s employment experience in laboratory work or equivalent training (e.g., one year of college science). Work requires the ability to follow detailed instructions and keep accurate records
- Bachelor’s degree in the biological sciences
- At least one year’s laboratory experience including familiarity with many or all of the relevant laboratory techniques
- Willingness to work with animals (mouse)
- Strong organizational skills
Final offers of employment are contingent upon successful completion of national criminal background check, national sex offender registry search and, where applicable, driving record.
Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of the job.