Research Technician I, Neurobiology
Research Tech I position at Duke Viral Vector Core
The Duke Viral Vector Core is a state-of-the art facility that provides comprehensive set of viral services to the Department of Neurobiology and Duke research community. We are currently looking for a Research Tech I, who will help us to support ongoing service programs.
Essential Duties and Responsibilities
Design and exercise various strategies for engineering and cloning new viral constructs based on AAV and Lentiviruses. Generate/expand a collection of DREADDs and other optogenetic-expressing constructs and vectors. Carry out molecular cloning and DNA manipulation, and additional molecular biology tasks to support ongoing service and research programs of the core.
Assume responsibility for production, QC/sequencing, tittering, and tracking of plasmids and vectors. This will involve preparation of DNA for large-scale vector production using commercially available kits, evaluation of their concentration and purity and routine tissue culture work. Assist in general service operations of the Duke VVC.
Communicate effectively with the PI and other members of the team. The person will establish work flow with the help of principle investigator and expected to execute procedures and experiments independently with minimal supervision. The person will be expected to supervise and help the work of lower level employees, such as students.
Preferred Qualifications, Education/Experience
BA degree or equivalent; previous experience producing constructs for viral vector production is a plus. Experience with molecular biology techniques is a plus, that includes: PCR, restriction digestion & cloning, primer design, plasmid isolation and analysis, bacterial transformation and screening, extraction of genomic DNA, DNA sequencing and analysis. Accompanying data analysis software proficiency is desired but not required.
Requisition Number 401501637
Duke Entity MEDICAL CENTER
Job Code 942 RESEARCH TECHNICIAN I
Job Family Level 06
Full Time / Part Time FULL TIME
Regular / Temporary Regular
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
Work requires an Associate's degree in botany, biology, zoology orother science related scientific field.
None required above education/training requirementOR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
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