Under the general supervision of a faculty investigator or laboratory supervisor, the Research Technologist will focus on performing both routine molecular biology assays and experimental techniques in the study of hepatitis C virus (HCV) and hepatitis B virus (HBV). He/she will be expected to organize and present data to the faculty investigator. Additional responsibilities will include contributing to general maintenance of the laboratory, equipment, and supplies.
Duties & Responsibilities
- Follow established procedures and utilizes aseptic technique to perform experiments associated with HCV and HBV virology and molecular biology. Includes but not limited to:
- Perform routine molecular biology assays to explore the viral and immune mechanisms underlying HBV and HCV infection including nucleic acid extraction, PCR, gel electrophoresis, RT-qPCR, basic histology techniques, and the operation of a laser-capture microdissection microscope.
- Assist with the collection and maintenance of a repository containing liver biopsies, PBMC, and plasma.
- Additional skills desired: Experience with Microsoft Office applications a plus.
- Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, scintillation counter, light microscope, plate washer and/or utilize biological safety cabinet. This person may also utilize sophisticated instrumentation such as flow cytometer, spectrophotometer, luminometer, thermal cycler and laboratory automation solutions/robotics. Responsible for operation of lab computers for data entry.
- Maintain accurate data records and laboratory notebook. Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Along with the laboratory supervisor, works to develop and institute protocols for routine laboratory analyses
Bachelor’s degree in biology, chemistry, or related field required.
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at firstname.lastname@example.org. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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