Research Specialist A, Pathology
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We seek an individual with extensive experience in cell and molecular biology with some animal experience coupled with the ability to oversee execution, analysis and interpretion of experiments investigating the mechanisms underlying the neuronal responses to neuroinflamation and neurotoxins associated with HIV infection of the Central nervous system. Experiments will investigate the ability of a investigational compound to induce the endogenous antioxidant response pathway and resume neurons from cell death, oxidative stress, changes in neurite outgrowth, and endoplasmic reticulum stress and the ability of the same compound to attenuate
HIV replication in primary human macrophages. Primary rat neuroglial cultures and primary human monocyte derived macrophages will be used for these studies. Molecular and cellular approaches will be used to assess the role of ER stress, the endogenous antioxidant response, and cell death pathways in neurons or macrophages responding to HIV infection of macrophages. Priority will be given to those who have experience with primary neuronal culture,immunofluorescent staining, confocal microscopy, handling of HIV-infected cells, qPCR, immunoblotting, transient transfection, and animal handling.
Perform protein chemistry analysis, work on primary neuronal culture, immunofluorescent staining, confocal microscopy, handling of HIV-infected cells, qPCR, immunoblotting, transient transfection, and animal handling. Uses scientific professional knowledge, techniques, and methods to assist Principal Investigator in carrying out projects to meet the research goals of the lab. Reflect competencies and behaviors of service orientation, accountability, adaptability, and taking initiative.
Performs a wide range of complex procedures encompassing nucleic acid, and protein analyses from influenza viruses, tissue culture, or primary clinical material. Conducts research under the guidance of the Principal Investigator, including planning, and conducting of experiments, data analysis and peer reviewed publication of data. Perform molecular biology, microbiological and molecular analytical techniques.
Serves as a laboratory support specialist and uses scientific professional knowledge, techniques, and methods to assist an Investigator in carrying out projects to meet the research goals of the lab. Work cooperatively with other PIs and staff in the laboratory to carry out collaborative research. Assist with other laboratory activities; including sample collection, sample preparation, solution preparation, performance of assays.
Responsible for quality control of the laboratory including upkeep and routine maintenance of equipment. Orders and maintains laboratory supplies and equipment including chemicals, biological materials, liquid nitrogen, etc. Required to perform computer analysis of data and use computer and computer software to prepare data for publication.
Prepares various media, stock supplies, and other reagents for use in the laboratory. Assists in the analysis and interpretation of data. Prepares summaries of laboratory findings to be used in reports and papers to be presented at scientific meetings and/or publication in scientific journals. Actively participates in the preparation of manuscripts, abstracts, and/or posters.
Bachelors Degree and 2 years to 3 years relevant experience required. Experience with molecular and cellular approaches in primary neuronal culture is essential. Ability to work with HIV infected cells is also essential. Priority will be given to those who have experience with primary neuronal culture, immunofluorescent staining, confocal microscopy, handling of HIV-infectedc cells, qPCR, immunoblotting, transient transfection, and animal handling.
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