Research Specialist A, Pathology

Location
Pennsylvania United States
Posted
11 Oct 2018
End of advertisement period
11 Dec 2018
Ref
51-30012
Contract Type
Permanent
Hours
Full Time

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn’s distinctive interdisciplinary approach to scholarship and learning.

Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play.

The University offers a competitive benefits package that includes excellent healthcare and tuition benefits for employees and their families, generous retirement benefits, a wide variety of professional development opportunities, supportive work and family benefits, a wealth of health and wellness programs and resources, and much more.

Established in 1878, Penn Dental Medicine is among the oldest university-affiliated dental schools in the nation. Penn Dental Medicine (PDM) is on a mission to provide the highest quality clinical and research environment for students while shaping the future of care. Join Penn Dental Medicine to live that mission every day by supporting the research and clinical needs of our patients, students, faculty, staff, and community. With PDM, not only can you maximize your career potential at an Ivy League Institution, but you can make a real difference in the lives of our patients and students. http://www.dental.upenn.edu/

We seek an individual with extensive experience in cell and molecular biology with some animal experience coupled with the ability to oversee execution, analysis and interpretion of experiments investigating the mechanisms underlying the neuronal responses to neuroinflamation and neurotoxins associated with HIV infection of the Central nervous system. Experiments will investigate the ability of a investigational compound to induce the endogenous antioxidant response pathway and resume neurons from cell death, oxidative stress, changes in neurite outgrowth, and endoplasmic reticulum stress and the ability of the same compound to attenuate

HIV replication in primary human macrophages. Primary rat neuroglial cultures and primary human monocyte derived macrophages will be used for these studies. Molecular and cellular approaches will be used to assess the role of ER stress, the endogenous antioxidant response, and cell death pathways in neurons or macrophages responding to HIV infection of macrophages. Priority will be given to those who have experience with primary neuronal culture,immunofluorescent staining, confocal microscopy, handling of HIV-infected cells, qPCR, immunoblotting, transient transfection, and animal handling.

Perform protein chemistry analysis, work on primary neuronal culture, immunofluorescent staining, confocal microscopy, handling of HIV-infected cells, qPCR, immunoblotting, transient transfection, and animal handling. Uses scientific professional knowledge, techniques, and methods to assist Principal Investigator in carrying out projects to meet the research goals of the lab. Reflect competencies and behaviors of service orientation, accountability, adaptability, and taking initiative.

Performs a wide range of complex procedures encompassing nucleic acid, and protein analyses from influenza viruses, tissue culture, or primary clinical material. Conducts research under the guidance of the Principal Investigator, including planning, and conducting of experiments, data analysis and peer reviewed publication of data. Perform molecular biology, microbiological and molecular analytical techniques.

Serves as a laboratory support specialist and uses scientific professional knowledge, techniques, and methods to assist an Investigator in carrying out projects to meet the research goals of the lab. Work cooperatively with other PIs and staff in the laboratory to carry out collaborative research. Assist with other laboratory activities; including sample collection, sample preparation, solution preparation, performance of assays.

Responsible for quality control of the laboratory including upkeep and routine maintenance of equipment. Orders and maintains laboratory supplies and equipment including chemicals, biological materials, liquid nitrogen, etc. Required to perform computer analysis of data and use computer and computer software to prepare data for publication.

Prepares various media, stock supplies, and other reagents for use in the laboratory. Assists in the analysis and interpretation of data. Prepares summaries of laboratory findings to be used in reports and papers to be presented at scientific meetings and/or publication in scientific journals. Actively participates in the preparation of manuscripts, abstracts, and/or posters.

Bachelors Degree and 2 years to 3 years relevant experience required. Experience with molecular and cellular approaches in primary neuronal culture is essential. Ability to work with HIV infected cells is also essential. Priority will be given to those who have experience with primary neuronal culture, immunofluorescent staining, confocal microscopy, handling of HIV-infectedc cells, qPCR, immunoblotting, transient transfection, and animal handling.

Penn adheres to a policy that prohibits discrimination on the basis of race, color, sex, sexual orientation, gender identity, religion, creed, national or ethnic origin, citizenship status, age, disability, veteran status, or any other legally protected class.