Life Science Research Professional
The Porteus Lab in the Department of Pediatrics, Division of Stem Cell Transplantation & Regenerative Medicine is seeking a Life Science Research Professional 1 (100% FTE) to perform basic functions and activities involved in defined research projects, and independently conduct and analyze experiments.
The Porteus laboratory employs genome editing techniques (CRISPR/cas9 and AAV) in primary various stem and progenitor cells for therapeutic applications. In this project, we are developing methods to correct mutations that cause cystic fibrosis in airway stem cells. The individual will perform day-to-day experiments under the guidance of Dr. Porteus, supporting isolation of cells from airway tissue, molecular biology, cell culture of airway stem cells, differentiation of airway stem cells in air-liquid interface and possibly some animal work to accomplish the goals of the project. This work will require the maintenance and expansion of primary human cells and include the preparation of DNA, RNA, and protein from the samples. S/he will be expected to collect, analyze, and present research data to the research group and make original scientific contributions to the research field. The LSRP may be expected to troubleshoot existing protocols and develop novel protocols and procedures and provide technical and scientific advice to other research personnel in lab. The position will also entail some lab support tasks such as ordering supplies and equipment, maintaining lab records, and adhering to safety regulations.
- Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
- Independently conduct experiments; maintain detailed records of experiments and outcomes.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
- Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
- Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assigned
- Bachelor’s or higher level degree in biology or related scientific field
- 1 year of lab experience
- Experience with CRISPR, AAV6 genetic techniques
- Proficiency in scientific writing in English
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree inrelated scientific field.
KNOWLEDGE, SKILLSAND ABILITIES (REQUIRED):
- General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge ofscience principals to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, usetelephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and filepaperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.