RESEARCH SCIENTIST, Biological Engineering-Synthetic Biology Center (SBC)-Weiss Lab, to lead a group focused on the development of an automated programmable organoid platform using a synthetic biology approach to genetically engineer liver organoids to rapidly advance the field of tissue maturation and drug development. Will genetically engineer self-organizing tissues from human pluripotent stem cells into liver organoids, validate their maturation properties, and use these mature organoids for drug testing; evaluate liver organoid performance by introducing additional factors that support hepatocytes maturation, either genetically or supplemented in the media; develop genetic circuit-based differentiation methods for programmed liver maturation, design and test genetic circuits that can detect distinct hepatic cell states and activate the expression of hepatics-specific developmental factors, leading to a functional and mature liver state; and develop gene circuits that can monitor miRNA and protein expression profiles to assess the responses of cellular pathways to drug compounds.
REQUIRED: Ph.D.-level scientist; five-plus years’ relevant experience in synthetic biology or related field; extensive knowledge of imaging technics, including flow cytometry, confocal, and immunofluorescence imaging; ability to master cell biology technics including transient and stable transfection of mammalian cell lines, RNAi and miRNA gene knock-down, DNA cloning, sub-cloning, PCR, RT-PCR, qPCR, and generation of protein expression vectors; experience with primary, 3D (spheres), and organoids cell culture; experience with molecular biology technics including site-directed and deletion mutagenesis, DNA, and RNA isolation; extensive cellular engineering expertise for generation of stable and inducible gene expression; demonstrated success leading research teams and working with industry collaborators; strong leadership/managerial/mentoring and scientific communication skills; ability to work collaboratively and independently; and a history of publishing in high-impact journals.
Must be able to work occasional nights and weekends.
MIT is an equal employment opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, sex, sexual orientation, gender identity, religion, disability, age, genetic information, veteran status, ancestry, or national or ethnic origin.