Department Konstantinos Tripsianes Research Group – Central European Institute of Technology
Deadline 30 Sep 2021
Start date Immediately or upon agreement

TWO positions in structural biology of Wnt signaling

CENTRAL EUROPEAN INSTITUTE OF TECHNOLOGY (CEITEC), BRNO, CZ

Research Group: Protein-DNA interactions

Research Group Leader: Konstantinos Tripsianes, Ph.D.

Project information:

We apply structural biology methods in order to gain a mechanistic view of CK1ε action in the Wnt signalling pathways. CK1ε represents an attractive therapeutic target but currently two key steps in the CK1ε-mediated Wnt signal transduction are unclear: (Project 1) how CK1ε gets activated and/or engages target proteins in response to Wnt signal and (Project 2) how CK1ε phosphorylates its key substrate DVL and what are the consequences of DVL phosphorylation.

We apply methods of integrated structural biology to (i) probe the DVL conformational landscape using in vitro and in vivo FRET sensors coupled to SAXS and CryoEM, (ii) understand the (auto)phosphorylation regulatory mechanisms of CK1ε, (iii) analyse by NMR the functional consequences of DVL phosphorylation and (iv) monitor DVL phosphorylation by real-time NMR under controlled cellular conditions.

Both positions (PhD or PostDoc level) are part of a multidisciplinary project that combines (i) cellular and molecular biology, (ii) proteomic analysis, (iii) biochemistry and structural biology, and received generous funding in a very competitive grant scheme.

Requirements:

We are looking for highly motivated researchers with a strong background or interest in structural biology. Hands-on experience with protein expression and purification is prerequisite. Experience with biomolecular NMR is a plus.

We offer:

• Salary that will be based on the candidate’s qualifications and experience.
• Background of a recognized and successful institution with supportive and international working environment
• Support with the relocation process (welcome office service)
• 6 weeks of paid holiday

Anticipated start date: Immediately or upon agreement.

Please send application (including CV, short statement of research background/interests and the contact information of at least two references) or queries by email to: kostas.tripsianes@ceitec.muni.cz

Electronic application deadline is: September 30th 2021