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The Research Assistant will be in charge of generating transgenic constructs and testing them in cell culture, genotyping, embryo and stem cell culture, in a project focused on investigation of cell fate.
- molecular cloning of transgenic constructs
- analysis of transgenic embryos
- cell culture
- Molecular cloning: PCR, restriction enzymes, gel electrophoresis, bacteriological technique
- Cell culture: transfection, passage
- Embryo culture, Imaging
- Experience with cell or embryo biology and/or molecular cloning, stem cell culture, transfection into cell lines