MASARYK UNIVERSITY

Laboratory Technician, RNA-based Regulation of Gene Expression

Location
Brno, Czech Republic
Posted
Thursday, 1 July 2021
End of advertisement period
Thursday, 30 September 2021
Ref
63817
Contract Type
Permanent

Department Peter Lukavsky Research Group Central European Institute of Technology
Deadline 30 Sep 2021
Start date negotiable

A position for a laboratory technician/manager is available in the research group of Peter J Lukavsky, PhD “RNA-based Regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU).  We study disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.

Job description:

  • Expression and purification of proteins
  • Transcription and purification of RNAs
  • Preparation of various media and buffers PCR and cloning of RNA and protein constructs
  • Preparation of isotope-labelled nucleotides
  • Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis
  • Performance of NMR experiments to study small molecule interactions with  proteins and RNA
  • Implementing new techniques and projects
  • Help incoming co-workers into a smooth start in the lab
  • Ordering of chemicals and lab supplies

Requirements:

  • At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences
  • Good knowledge of English (oral and written)
  • Excellent interpersonal and communication skills
  • High motivation, flexibility and self-driven interest to learn new things
  • Reliability, team player, meticulousness
  • Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.
  • Computer literacy, MS word, Excel, Adobe Illustrator.
  • Excellent experience with molecular biology, protein and RNA purification, biochemistry and NMR spectroscopy is a big plus

We offer:

  • Interesting work in a top scientific team
  • Stable base and excellent instrumental equipment of CEITEC-MU MU employment benefits
  • Salary based on qualification and experience; part time appointment possible

Brno is an attractive, lively place to live and work with many cultural and recreational amenities.

Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references to Peter Lukavsky (peter.lukavsky@ceitec.muni.cz).  Candidates considered for the second round of interview will be informed via email.

Deadline of the applications is 30 September 2021.

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