KINGS COLLEGE LONDON

Research Technician – Leukemia Epigenetics and DDR

Location
London (Greater)
Salary
£28,717 - £31,831, including London Weighting Allowance
Posted
Tuesday, 8 June 2021
End of advertisement period
Sunday, 27 June 2021
Ref
024247
Academic Discipline
Life sciences, Biological Sciences
Contract Type
Fixed Term
Hours
Full Time

Job description
The purpose of this post is to develop and expedite projects aimed at characterizing leukemia networks in leukemia using newly developed disease models in the lab.  Transcription factors and their associated epigenetic modifying enzymes are frequently deregulated or even mutated in human acute myeloid leukemia (Zeisig et al., 2012, Cancer Cell).  Over the years, our lab has focused on characterization of key transcriptional/epigenetic networks and their emerging crosstalk with DNA damage repair (DDR) mechanisms critical for leukemia development (Kwok et al., 2006, Cancer Cell; Zeisig et al., 2007, Cancer Cell; Cheung et al., 2007, Nat Cell Biology; Arteaga et al., 2013, Cancer Cell; Esposito et al., 2015, Nature Medicine; Cheung et al., 2016, Cancer Cell; Luo et al., 2019, Cancer Cell). Details about the lab can be found at https://www.kcl.ac.uk/research/leukaemia-and-stem-cell-biology. Candidates are expected to have strong background and relevant publications in areas of DDR, epigenetic gene regulation and/or cancer biology.
 
This is a research post aiming to assist Professor So in the design and expediting of his research projects for publication of academic papers and securing of grants.


The post holder will:

  • To help to maintain the general operation of the lab
  • To introduce appropriate new techniques and procedures, ensuring that all new techniques and procedures techniques are adequately validated and documented and ensure that colleagues are trained in new techniques and procedures.
  • To directly participate and drive research projects

This post will be offered on an a fixed-term contract for 24 months
This is a full-time post - 100% full time equivalent

Key responsibilities
Analysis & Research:
o   FACS sorting and analysis
o   Tissue culture and cell transformation assay
o   In vivo disease modelling using genetically modified mice
o   working and characterizing of human patient samples
o   Immunofluorescent staining
o   Transcriptomic (e.g. RNA-seq) analysis.
o   ChIP-Seq, MeDIP-Seq analysis
o   Leukemia and stem cell biology studies

  • providing assistance on a day-to-day basis according to the ever-changing needs of the Group as directed by the Head of the Leukaemia and Stem Cell Biology Group. This will require a commitment to a very flexible and service-oriented / team-working approach
  • introduce appropriate new techniques and procedures, ensure that all new techniques and procedures techniques are adequately validated and documented and ensure that colleagues are trained in new techniques and procedures.
  • design and implement systems for laboratory information management (both electronic and manual) and archiving of analytical material.
  • develop original novel research projects based on thorough assessment of the published literature and the body of knowledge within their own speciality.

Team Work, Teaching & Learning Support: 

  • providing assistance on a day-to-day basis according to the ever-changing needs of the Group as directed by the Head of the Leukaemia and Stem Cell Biology Group. This will require a commitment to a very flexible and service-oriented / team-working approach
  • be trained in procedures as required by more senior and more experienced members of the Group
  • assist with the training of new members of staff and students in the establishment procedures of the Group
  • develop and improve their own scientific knowledge and awareness of developments within the field of leukaemia stem cell biology via attendance of relevant meetings and conferences.

Supervision, Communication & Networking:

  • assist in grant application preparation, project justifications, business cases and project reports
  • prepare manuscripts for publication in peer reviewed academic journals
  • communicate in a timely fashion any adverse event or condition which will/may cause failure or disruption of the Group’s activities to the Head of the Leukaemia Stem Cell Biology Group
  • form effective networks with colleagues in other Groups of the department, with peers in King’s College London at large and outside of the college to assist the Head of the Leukaemia Stem Cell Biology Group to form and develop collaborative links
  • maintain professional conduct with all members of staff and visitors at all times.
  • communicate with suppliers for procurement of goods and services and in trouble-shooting problems with supplies and equipment.

Decision Making, Planning and Problem Solving:

  • be accountable to the Head of Department
  • report to the Head of the Leukaemia and Stem Cell Biology Group for goal setting and prioritisation of workload
  • prioritise and plan own workload and communicate own work priorities within the team.
  • undertake complex laboratory tasks, including high-level problem solving, such as rectification of failures in laboratory equipment.

Service Delivery:

  • assist the Head of the Leukaemia and Stem Cell Biology Group in agreeing, defining and managing service standards

Sensory/Physical Demands & Work Environment:

  • adhere to the Departmental Code of Safe Working Practice at all times
  • assist the Head of the Leukaemia and Stem Cell Biology Group to maintain a safe working environment by acting as a Room Supervisor for one or more rooms within the Group’s facilities if and when directed by the Head of the Group
  • be required to work within the confined space of Biological/Microbiological Safety Cabinets and to carry out procedures that demand high levels of concentration for long periods of time

Skills, knowledge, and experience


Essential criteria 
•       BSc Hons in a biomedical sciences subject or equivalent (eg medical degree)
•       A thorough knowledge of leukemia or hematopoietic stem cell biology
•       Competent in the interpretation and technical validation of results
•       Good verbal and written communication skills in English
•       Ability to think logically, adapt to changing requirements  and use initiative
•       Ability to work to deadlines under pressure
•       Ability to work as part of a team and independently
•       Ability to organise workload and to respond accordingly to changes in the workload
•       Ability to manipulate laboratory computer systems and standard software packages
•       Experience of work with biological samples, laboratory information systems
•       Experience in developing new assays and procedures
 
Desirable criteria 
•       Master or a higher degree in which investigations of epigenetic regulation/DDR/Biochemistry formed a substantial part.
•       Relevant publication in peer-reviewed journal
•       Experience in the study of transcriptional / epigenetic processes
•       Experience of procedures used in the characterisation of haemopoiesis, including xenotransplantation models
•       Experiences in handling mouse or maintenance of colony