Technical Associate, Weiss Lab for Synthetic Biology

6 days left

Location
Massachusetts, United States
Posted
Sunday, 21 June 2020
End of advertisement period
Friday, 21 August 2020
Ref
16905
Contract Type
Permanent
Hours
Full Time

Working at MIT offers opportunities, an environment, a culture – and benefits – that just aren’t found together anywhere else. If you’re curious, motivated, want to be part of a unique community, and help shape the future – then take a look at this opportunity.

TECHNICAL ASSOCIATE, Biological Engineering-Synthetic Biology Center (SBC) (multiple positions), to join the lab of Professor Ron Weiss. Will aid in the development and production of DNA-based genetic circuits and validate their sequences and properties; aid in the development, production, and titer measurements of lentivirus gene delivery vectors and their transduction into target cells and the management and characterization of cells undergoing the reprogramming process, cells that have achieved pluripotency, or the characterization of differentiating cells; and use mature organoids for drug testing and toxicology studies or assist with developing and testing gene circuits that can monitor miRNA and protein expression profiles to assess the responses of cellular pathways to drug compounds. Laboratory work includes mammalian and/or organoid cell culture, molecular cloning (genetic engineering), transfection, lentivirus production, lentivirus transduction, immunofluorescent staining, flow cytometry, microscopic image acquisition, ELISA, Droplet Digital PCR, and data preparation; preparing progress reports; and providing careful documentation of experimental procedures.

Job Requirements

REQUIRED: B.S./B.A. (master’s preferred) in biology, bioengineering, biochemistry, or other relevant scientific field; at least two year’s laboratory technician experience; experience with basic aseptic molecular and cellular biology and mammalian cell culture technique and basic chemistry/biology lab techniques; laboratory experience with basic experimental procedures; excellent organizational, planning, interpersonal, and written and verbal communication skills; self-motivation; detail-orientation; and ability to work independently and as part of a team, follow complex instructions, plan and troubleshoot experiments, multitask, and carefully document work in a fast-paced and dynamic research environment. Experience with DNA and RNA handling/transfection, lentivirus production and transduction, stem cell culture, and organoid culture desirable; as is knowledge of imaging technique, including flow cytometry and confocal and immunofluorescence imaging. Job #16905

Some work requires use of lentiviruses in a BL2 or BL2+ setting.

Some nights and weekend work will be required.

MIT is an equal employment opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, sex, sexual orientation, gender identity, religion, disability, age, genetic information, veteran status, ancestry, or national or ethnic origin.

MIT considers equivalent combinations of experience and education for certain jobs. All candidates who believe they possess equivalent experience and education are encouraged to apply.

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